Impact and Persistence of Serratia marcescens in Tenebrio molitor Larvae and Feed under Optimal and Stressed Mass Rearing Conditions

Industrial insect mass rearing aims to produce quality insects under safe sanitary conditions which can be compromised by pathogens and abiotic stressors. Therefore, knowledge on pathogen persistence, virulence and means of detection is of importance. This study focuses on the opportunistic pathogen...

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Bibliographic Details
Published in:Insects (Basel, Switzerland) Switzerland), 2022-05, Vol.13 (5), p.458
Main Authors: Dupriez, Florent, Rejasse, Agnès, Rios, Alfredo, Lefebvre, Thomas, Nielsen-LeRoux, Christina
Format: Article
Language:English
Subjects:
Abiotic stress
Animal feed
Animal husbandry
Bacteria
Culture media
Density
Factorial experiments
Feces
Feeds
Food contamination & poisoning
food safety
Growth media
Industrial development
insect for food and feed
Insects
Kinetics
Larvae
Larval development
Life Sciences
Livestock
Mass production
Mass rearing
microbial persistence
Microbiota
Nosocomial infections
Opportunist infection
Pathogens
qPCR
Salmonella
Serratia
Serratia marcescens
Starvation
Survival
Tenebrio molitor
Virulence
yellow mealworm
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Summary:Industrial insect mass rearing aims to produce quality insects under safe sanitary conditions which can be compromised by pathogens and abiotic stressors. Therefore, knowledge on pathogen persistence, virulence and means of detection is of importance. This study focuses on the opportunistic pathogen ( ) as a possible candidate to reveal sanitary issues in ( ) breeding. A screening test was performed to assess the impact of abiotic stressors (starvation, density and sieving) in presence and absence of . Two detection methods were conducted, and the kinetics of persistence were investigated. Our results show that (i) the presence of had a low but significant effect on mortality, (ii) a short temporary starvation period had a negative impact on larval growth, (iii) the detection of by q-PCR was sensitive but less convenient than a specific growth media, (iv) the kinetics of persistence showed that declined but survived for nine days in the feed and in the feces for three weeks. Both the relatively low virulence and the persistence in the environment suggest that could be used as an indicator for the sanitary status of mealworm production.
ISSN:2075-4450
2075-4450
DOI:10.3390/insects13050458