Oncogenic zinc finger protein ZNF687 accelerates lung adenocarcinoma cell proliferation and tumor progression by activating the PI3K/AKT signaling pathway

Background Zinc finger protein 687 (ZNF687) has previously been discovered as a potential oncogene in individuals with giant cell tumors of the bone, acute myeloid leukemia, and hepatocellular carcinoma. However, its role and mechanism in lung adenocarcinoma (LUAD) remain unclear. Methods In LUAD ce...

Full description

Saved in:
Bibliographic Details
Published in:Thoracic cancer 2023-05, Vol.14 (14), p.1223-1238
Main Authors: Li, Mingchun, Liu, Zhihua, Hou, Zan, Wang, Xiangcai, Shi, Huaqiu, Li, Yamei, Xiao, Xuewen, Tang, Zhixian, Yang, Jianqiong, Luo, Yaoling, Zhang, Minhong, Chen, Ming
Format: Article
Language:English
Subjects:
Adenocarcinoma of Lung - pathology
Biotechnology industry
Cancer therapies
Cell cycle
Cell Line, Tumor
Cell Movement
Cell Proliferation
Chemotherapy
Cyclin-dependent kinases
EMT
Gene Expression Regulation, Neoplastic
Genes
Humans
Immunotherapy
Kinases
Liver cancer
lung adenocarcinoma
Lung cancer
Lung Neoplasms - pathology
Medical prognosis
Original
Phosphatidylinositol 3-Kinase - metabolism
Phosphatidylinositol 3-Kinases - genetics
Phosphatidylinositol 3-Kinases - metabolism
PI3K/ AKT
Protein expression
Proteins
Proto-Oncogene Proteins c-akt - metabolism
Radiation therapy
Signal Transduction
Tumors
Variance analysis
ZNF687
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Zinc finger protein 687 (ZNF687) has previously been discovered as a potential oncogene in individuals with giant cell tumors of the bone, acute myeloid leukemia, and hepatocellular carcinoma. However, its role and mechanism in lung adenocarcinoma (LUAD) remain unclear. Methods In LUAD cells, tumor, and matched adjacent tissue specimens, quantitative real‐time RT‐ polymerase chain reaction (qRT‐PCR), western blotting analyses, and immunohistochemistry staining (IHC) were conducted. Cell counting kit‐8 (CCK8) assay, clonogenicity analysis, flow cytometry, and transwell assays were utilized to detect ZNF687 overexpression and knockdown impacts on cell growth, colony formation, cell cycle, migration, and invasion. Bioinformatic studies, qRT‐PCR and western blotting studies were employed to validate the underlying mechanisms and signaling pathways implicated in the oncogenic effect of ZNF687. Results This study demonstrated that ZNF687 expression was elevated in LUAD cells and tissues. Individuals with upregulated ZNF687 had a poorer prognosis than those with downregulatedZNF687 (p 
ISSN:1759-7706
1759-7714
DOI:10.1111/1759-7714.14856