The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts

The introduction of exogenous molecules into embryos is required for analyses of molecular dynamics and specific gene functions during early embryonic development. Electroporation is an effective method to transport exogenous molecules into cells, but is rarely used in bovine embryos. First, we eval...

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Bibliographic Details
Published in:Journal of Reproduction and Development 2019, Vol.65(5), pp.475-479
Main Authors: TANIHARA, Fuminori, HIRATA, Maki, MORIKAWA, Shigeki, NGUYEN, Nhien Thi, LE, Quynh Anh, HIRANO, Takayuki, FUKUMI, Yoshiyuki, ABE, Toshiaki, OTOI, Takeshige
Format: Article
Language:English
Subjects:
Blastocyst
Blastocysts
Bovine
Electroporation
Embryogenesis
Fertility
Fluorescein
Hatching
In vivo
Oligonucleotides
Technology Report
Zona pellucida
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Summary:The introduction of exogenous molecules into embryos is required for analyses of molecular dynamics and specific gene functions during early embryonic development. Electroporation is an effective method to transport exogenous molecules into cells, but is rarely used in bovine embryos. First, we evaluated the viability of in vivo-derived bovine blastocysts after electroporation with fluorescein (FAM) labeled-oligonucleotides with varying pulse numbers (3, 5, 7, and 10), while keeping the pulse duration at 1 msec and the electric field of 20 V/mm. Next, we examined the effects of zona pellucida status on blastocyst quality after electroporation, by comparing the average diameter of blastocysts before and after electroporation using blastocysts with intact zona pellucida and hatching/hatched blastocysts. Electroporation successfully introduced exogenous molecules into in vivo-derived bovine blastocysts without loss of viability. Moreover, the status of the zona pellucida may be associated with the quality of blastocysts after electroporation.
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.2019-049