RNA-Seq transcriptome data of human cells infected with influenza A/Puerto Rico/8/1934 (H1N1) virus

Human influenza remains a serious public health problem. This data article reports the transcriptome analysis data of human cell lines infected with influenza A/Puerto Rico/8/1934 (H1N1) virus. Mock-infected cells were included as controls. Human embryonic fibroblasts (MRC-5) and immortalized cell l...

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Bibliographic Details
Published in:Data in brief 2020-12, Vol.33, p.106604-106604, Article 106604
Main Authors: Zhuravlev, Evgenii, Sergeeva, Mariia, Malanin, Sergey, Amirkhanov, Rinat, Semenov, Dmitriy, Grigoryeva, Tatiana, Komissarov, Andrey, Stepanov, Grigory
Format: Article
Language:English
Subjects:
H1N1
Human cells
Influenza A virus
Microbiology
RNA-seq
Transcriptome
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Summary:Human influenza remains a serious public health problem. This data article reports the transcriptome analysis data of human cell lines infected with influenza A/Puerto Rico/8/1934 (H1N1) virus. Mock-infected cells were included as controls. Human embryonic fibroblasts (MRC-5) and immortalized cell lines (A549, HEK293FT, WI-38 VA-13) were selected for RNA sequencing using Illumina NextSeq500 platform. Raw data were applied to the bioinformatic pipeline, which includes quality control with FastQC and MultiQC, adapter and quality trimming with Cutadapt, filtering to the genome of influenza A with STAR, transcript quantification with Salmon tool (GRCh38_RefSeq_Transcripts). Differential expressed genes were identified using R package DESeq2 with FDR-adjusted p-value < 0.001 and absolute value of log2(FC) > 1. Lists of differentially expressed genes is provided. The raw and processed RNA-seq data presented in this article were deposited to the European Nucleotide Archive via the ArrayExpress partner repository with the dataset accession number E-MTAB-9511 .
ISSN:2352-3409
2352-3409
DOI:10.1016/j.dib.2020.106604