Evaluation of antimicrobial and cytotoxic activities of plant extracts from southern Minas Gerais cerrado

• Published in: Revista do Instituto de Medicina Tropical de São Paulo 2014-01, Vol.56 (1), p.13-20
• Main Authors: Chavasco, Juliana Moscardini, Prado E Feliphe, Bárbara Helena Muniz, Cerdeira, Claudio Daniel, Leandro, Fabrício Damasceno, Coelho, Luiz Felipe Leomil, Silva, Jéferson Junior da, Chavasco, Jorge Kleber, Dias, Amanda Latercia Tranches
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antifungal Agents - isolation & purification; Antifungal Agents - pharmacology; Antimicrobial activity; Brazil; Cytotoxicity; Fungi - drug effects; Gram-Negative Bacteria - drug effects; Gram-Positive Bacteria - drug effects; Microbial Sensitivity Tests; Mycobacterium - drug effects; Mycobacterium bovis; Mycobacterium tuberculosis; Plant Antimicrobial Activity; Plant extracts; Plant Extracts - classification; Plant Extracts - pharmacology; TROPICAL MEDICINE
• ISSN: 0036-4665; 1678-9946; 1678-9946; 0036-4665
• DOI: 10.1590/S0036-46652014000100002

The antimicrobial activity of plant hidroethanolic extracts on bacteria Gram positive, Gram negative, yeasts, Mycobacterium tuberculosis H37 and Mycobacterium bovis was evaluated by using the technique of Agar diffusion and microdilution in broth. Among the extracts evaluated by Agar diffusion, the extract of Bidens pilosa leaf presented the most expressive average of haloes of growth inhibition to the microorganisms, followed by the extract of B. pilosa flower, of Eugenia pyriformis' leaf and seed, of Plinia cauliflora leaf which statistically presented the same average of haloes inhibitory formation on bacteria Gram positive, Gram negative and yeasts. The extracts of Heliconia rostrata did not present activity. Mycobacterium tuberculosis H37 and Mycobacterium bovis (BCG) appeared resistant to all the extracts. The susceptibility profile of Candida albicans and Saccharomyces cerevisiae fungi were compared to one another and to the Gram positive Bacillus subtilis, Enterococcus faecalis and the Gram negative Salmonella typhimurium bacteria (p > 0.05). The evaluation of cytotoxicity was carried out on C6-36 larvae cells of the Aedes albopictus mosquito. The extracts of stem and flower of Heliconia rostrata, leaf and stem of Plinia cauliflora, seed of Anonna crassiflora and stem, flower and root of B. pilosa did not present toxicity in the analyzed concentrations. The highest rates of selectivity appeared in the extracts of stem of A. crassiflora and flower of B. pilosa to Staphylococcus aureus, presenting potential for future studies about a new drug development.