Efficacy of microbicidal actives and formulations for inactivation of Lassa virus in suspension

The World Health Organization’s R&D Blueprint list of priority diseases for 2022 includes Lassa fever, signifying the need for research and development in emergency contexts. This disease is caused by the arenavirus Lassa virus (LASV). Being an enveloped virus, LASV should be susceptible to a va...

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Published in:Scientific reports 2023-08, Vol.13 (1), p.12983-12983, Article 12983
Main Authors: Cutts, Todd A., Nims, Raymond W., Rubino, Joseph R., McKinney, Julie, Kuhn, Jens H., Ijaz, M. Khalid
Format: Article
Language:English
Subjects:
631/326/22
631/326/421
631/326/596
Ammonium
Bovine serum albumin
Disinfectants
Disinfection & disinfectants
Ethanol
Fever
Green fluorescent protein
Humanities and Social Sciences
Hydrogen peroxide
Inactivation
Laboratories
Lassa fever
Microbicides
multidisciplinary
Proteins
Public health
R&D
Research & development
Science
Science (multidisciplinary)
Sodium hypochlorite
Vero cells
Viruses
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Summary:The World Health Organization’s R&D Blueprint list of priority diseases for 2022 includes Lassa fever, signifying the need for research and development in emergency contexts. This disease is caused by the arenavirus Lassa virus (LASV). Being an enveloped virus, LASV should be susceptible to a variety of microbicidal actives, although empirical data to support this expectation are needed. We evaluated the virucidal efficacy of sodium hypochlorite, ethanol, a formulated dual quaternary ammonium compound, an accelerated hydrogen peroxide formulation, and a p -chloro- m -xylenol formulation, per ASTM E1052-20, against LASV engineered to express green fluorescent protein (GFP). A 10-μL volume of virus in tripartite soil (bovine serum albumin, tryptone, and mucin) was combined with 50 μL of disinfectant in suspension for 0.5, 1, 5, or 10 min at 20–25 °C. Neutralized test mixtures were quantified by GFP expression to determine log 10 reduction. Remaining material was passaged on Vero cells to confirm absence of residual infectious virus. Input virus titers of 6.6–8.0 log 10 per assay were completely inactivated by each disinfectant within 1–5 min contact time. The rapid and substantial inactivation of LASV suggests the utility of these microbicides for mitigating spread of infectious virus during Lassa fever outbreaks.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-38954-5