The Structural Interactions of Molecular and Fibrillar Collagen Type I with Fibronectin and Its Role in the Regulation of Mesenchymal Stem Cell Morphology and Functional Activity

The observed differences in the structure of native tissue and tissue formed in vitro cause the loss of functional activity of cells cultured in vitro. The lack of fundamental knowledge about the protein mechanism interactions limits the ability to effectively create in vitro native tissue. Collagen...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-10, Vol.23 (20), p.12577
Main Authors: Nashchekina, Yuliya, Nikonov, Pavel, Prasolov, Nikita, Sulatsky, Maksim, Chabina, Alina, Nashchekin, Alexey
Format: Article
Language:English
Subjects:
actin cytoskeleton
Cell adhesion & migration
cell focal contacts
Cell morphology
Collagen
Collagen (type I)
Composition effects
Confocal microscopy
Cytology
Extracellular matrix
Fibrils
Fibronectin
Fibrous structure
Mesenchymal stem cells
Microscopy
molecular and fibrillar collagens
Molecular structure
Physiology
Protein composition
Protein structure
Proteins
Stem cells
Transmission electron microscopy
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Summary:The observed differences in the structure of native tissue and tissue formed in vitro cause the loss of functional activity of cells cultured in vitro. The lack of fundamental knowledge about the protein mechanism interactions limits the ability to effectively create in vitro native tissue. Collagen is able to spontaneously assemble into fibrils in vitro, but in vivo, other proteins, for example fibronectin, have a noticeable effect on this process. The molecular or fibrillar structure of collagen plays an equally important role. Therefore, we studied the interaction of the molecular and fibrillar structure of collagen with fibronectin. Atomic force and transmission electron microscopy showed that the presence of fibronectin does not affect the native structure and diameter of collagen fibrils. Confocal microscopy demonstrated that the collagen structure affects the cell morphology. Cells are better spread on molecular collagen compared with cells cultured on fibrillar collagen. Fibronectin promotes the formation of a large number of focal contacts, while in combination with collagen of both forms, its effect is leveled. Thus, understanding the mechanisms of the relationship between the protein structure and composition will effectively manage the creation in vitro of a new tissue with native properties.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms232012577