Chemical Characterization and Metagenomic Identification of Endophytic Microbiome from South African Sunflower ( Helianthus annus ) Seeds

• Published in: Microorganisms (Basel) 2023-04, Vol.11 (4), p.988
• Main Authors: Balogun, Fatai Oladunni, Abdulsalam, Rukayat Abiola, Ojo, Abidemi Oluranti, Cason, Errol, Sabiu, Saheed
• Format: Article
• Language: English
• Subjects: Abundance; Agricultural production; Agrochemicals; Alternaria; Analysis; Bacillus; Bacteria; bacteria diversity; Biological control; Chromatography; Composition; Crop diseases; Cultivars; Diseases and pests; DNA sequencing; endophyte; Endophytes; Fatty acids; Fungi; fungi diversity; Fungicides; Genetic aspects; Growth; Helianthus; Linoleic acid; Metagenomics; microbiome; Microbiomes; Microorganisms; next-generation sequencing; Nucleotide sequencing; Oils & fats; Oilseed crops; Oilseeds; Pathogens; Pesticides; Pests; Plant growth; Relative abundance; rRNA; Seeds; sunflower seeds; Sunflowers; Unsaturated fatty acids
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11040988

(sunflower) is a globally important oilseed crop whose survival is threatened by various pathogenic diseases. Agrochemical products are used to eradicate these diseases; however, due to their unfriendly environmental consequences, characterizing microorganisms for exploration as biocontrol agents are considered better alternatives against the use of synthetic chemicals. The study assessed the oil contents of 20 sunflower seed cultivars using FAMEs-chromatography and characterized the endophytic fungi and bacteria microbiome using Illumina sequencing of fungi ITS 1 and bacteria 16S (V3-V4) regions of the rRNA operon. The oil contents ranged between 41-52.8%, and 23 fatty acid components (in varied amounts) were found in all the cultivars, with linoleic (53%) and oleic (28%) acids as the most abundant. Ascomycota (fungi) and Proteobacteria (bacteria) dominated the cultivars at the phyla level, while and at the genus level in varying abundance. AGSUN 5102 and AGSUN 5101 (AGSUN 5270 for bacteria) had the highest fungi diversity structure, which may have been contributed by the high relative abundance of linoleic acid in the fatty acid components. Dominant fungi genera such as , , , , , , and bacteria including , and are established, providing insight into the fungi and bacteria community structures from the seeds of South Africa sunflower.