Circular RNA Circ-ZNF609 Promotes Lung Adenocarcinoma Proliferation by Modulating miR-1224-3p/ETV1 Signaling

Circ-ZNF609 has been shown to modulate cancer progression in several kinds of cancer. However, the role of circ-ZNF609 played in lung adenocarcinoma (LAUD) remains unclear. In this study, we investigated the role of circ-ZNF609 in regulating LAUD cancer progression. Quantitative reverse transcriptio...

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Bibliographic Details
Published in:Cancer management and research 2020-01, Vol.12, p.2471-2479
Main Authors: Zuo, Yangsong, Shen, Wenyi, Wang, Chengshi, Niu, Niu, Pu, Juan
Format: Article
Language:English
Subjects:
Adenocarcinoma
Analysis
Binding sites
Cancer therapies
Cell cycle
Cell growth
cerna
circ-znf609
Development and progression
evt1
Gene expression
Health aspects
Journalists
Liver cancer
Luciferase
lung adenocarcinoma
Lung cancer
Medical prognosis
MicroRNAs
mir-1224-3p
Original Research
Polymerase chain reaction
RNA
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Summary:Circ-ZNF609 has been shown to modulate cancer progression in several kinds of cancer. However, the role of circ-ZNF609 played in lung adenocarcinoma (LAUD) remains unclear. In this study, we investigated the role of circ-ZNF609 in regulating LAUD cancer progression. Quantitative reverse transcription polymerase chain reaction was conducted to evaluate circ-ZNF609 expression in 52 LAUD tissues and 52 matched adjacent normal tissues, LAUD cell lines and bronchial epithelial cell line (HBE). The direct interaction between miR-1224-3p and circ-ZNF609 or EVT1 was verified using luciferase reporter assay and RNA immunoprecipitation assay. Cell Counting Kit-8, colony formation assay, cell-cycle analysis were utilized to examine the effect of circ-ZNF609 or miR-1224-3p on cell proliferation. Circ-ZNF609 was significantly upregulated in LAUD tissues and cell lines, and its inhibition induced reduced cell proliferation of LAUD cells. Mechanistically, we demonstrated that circ-ZNF609 sponged miR-1224-3p to promote EVT1 expression. More importantly, miR-1224-3p overexpression strongly suppressed circZNF609-induced malignant phenotype of LAUD cells. Circ-ZNF609 enhances LAUD progression by increasing oncogenic EVT1 expression via sponging miR-1224-3p, revealing that circ-ZNF609/miR-1224-3p/ETV1 axis may be a promising therapeutic target for LAUD treatment.
ISSN:1179-1322
1179-1322
DOI:10.2147/CMAR.S232260