UJI AKTIVITAS ANTIOKSIDAN FRAKSI ETER HASIL HIDROLISIS INFUSA DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) DENGAN METODE DPPH (1,1-DIPHENIL-2-PICRYLHYDRAZYL)

• Published in: Pharmaciana 2014-05, Vol.4 (1), p.1-8
• Main Authors: Ardianti, Agustina, Guntarti, Any, Zainab, Zainab
• Format: Article
• Language: English
• Subjects: antioxidant; Binahong (Anredera cordifolia (Ten.) Steenis); DPPH; free radical
• ISSN: 2088-4559; 2477-0256
• DOI: 10.12928/pharmaciana.v4i1.391

Antioxidant is compounds which have abilities to inhibit oxidation rate. Anredera cordifolia folium consist of flavonoid which have activities as antioxidant. The purpose of this research was to know antioxidant activity at ether fraction of hydrolysed aquous extract of Binahong folium (Anredera cordifolia). Ether fraction was obtained by hydrolysing the infuse Binahong folium (Anredera cordifolia) with HCl 2N then fractionated with ether and then evaporated. The antioxidant activity was measured by DPPH method using spectrophotometer. The maximum wavelength and operating time quercetin and samples were 515,0 nm (quersetin) and 514,0 nm (ether fraction), 19-30 minute (quersetin) and 19-29 minute (ether fraction). Scavenging activity test done with the DPPH method (1,1- dyphenyl-2-picrylhydrazyl). The decreasing of DPPH radical solution decreased the absorbant intensity. The percentage of radical scavenging activity was performed as EC50 (Effective Concentration). The results indicated that both samples showed free radical scavenging activity with EC50 value for quercetin (2,702±0,15) µg/ml, and ether fraction of (249,31±9,26) µg/ml. Statistical analysis using Kruskall Wallis method with 95% of confident level followed by Mann Whitney test giving evidence the results between samples and positive control (quercetin) are significantly difference.