Differential Effects of Mycobacterium bovis BCG on Macrophages and Dendritic Cells from Murine Spleen

Macrophages (MΦ) and dendritic cells (DCs) are both pivotal antigen presenting cells capable of inducing specific cellular responses to inhaled mycobacteria, and thus, they may be important in the initiation of early immune responses to mycobacterial infection. In this study, we evaluated and compar...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2015-10, Vol.16 (10), p.24127-24138
Main Authors: Xu, Zhengzhong, Meng, Chuang, Qiang, Bin, Gu, Hongyan, Sun, Lin, Yin, Yuelan, Pan, Zhiming, Chen, Xiang, Jiao, Xinan
Format: Article
Language:English
Subjects:
Animals
Antigen Presentation - immunology
Antigens
Antigens, Bacterial - immunology
Bacteria
Bacterial infections
BCG
Cells
Cytokines - immunology
dendritic cell
Dendritic Cells - immunology
Female
Green Fluorescent Proteins - genetics
Immune system
Lymphocyte Activation - immunology
macrophage
Macrophages - immunology
Mice
Mice, Inbred C57BL
Mycobacterium bovis
Mycobacterium bovis - genetics
Mycobacterium bovis - immunology
Nitric Oxide - immunology
Nitric Oxide Synthase Type II - immunology
Reactive Oxygen Species - immunology
Spleen - cytology
Spleen - immunology
T-Lymphocytes - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Macrophages (MΦ) and dendritic cells (DCs) are both pivotal antigen presenting cells capable of inducing specific cellular responses to inhaled mycobacteria, and thus, they may be important in the initiation of early immune responses to mycobacterial infection. In this study, we evaluated and compared the roles of murine splenic DCs and MΦs in immunity against Mycobacterium bovis Bacillus Calmette-Guérin (M.bovis BCG). The number of internalized rBCG-GFP observed was obviously greater in murine splenic MΦs compared with DCs, and the intracellular reactive oxygen species (ROS), inducible nitric oxide synthase (iNOS) and nitric oxide (NO) levels in MΦs were all higher than in DCs. DCs have a stronger capacity for presenting Ag85A peptide to specific T hybridoma and when the murine splenic MΦs were infected with BCG and rBCG::Ag85A, low level of antigen presenting activity was detected. These data suggest that murine splenic MΦs participate in mycobacteria uptake, killing and inducing inflammatory response, whereas the murine splenic DCs are primarily involved in specific antigen presentation and T cell activation.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms161024127