Evaluating stored platelet shape change using imaging flow cytometry

Platelets are routinely stored at room temperature for 5-7 days before transfusion. Stored platelet quality is traditionally assessed by Kunicki's morphology score. This method requires extensive training, experience, and is highly subjective. Moreover, the number of laboratories familiar with...

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Bibliographic Details
Published in:Platelets (Edinburgh) 2023-12, Vol.34 (1), p.2136646-2136646
Main Authors: Özpolat, Tahsin, Yakovenko, Olga, Stratiievska, Anastasiia, Bailey, S Lawrence, Miles, Jeffrey, Usaneerungrueng, Chomkan, Byrne, Daire, Wu, Xiaoping, Stolla, Moritz
Format: Article
Language:English
Subjects:
Animals
Blood Platelets
Blood Preservation
cold storage
Cold Temperature
Cryopreservation
Flow Cytometry
Humans
imaging flow cytometry
kunicki morphology score
Mice
Platelet Transfusion
platelets
shape change
Temperature
transfusion
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Summary:Platelets are routinely stored at room temperature for 5-7 days before transfusion. Stored platelet quality is traditionally assessed by Kunicki's morphology score. This method requires extensive training, experience, and is highly subjective. Moreover, the number of laboratories familiar with this technique is decreasing. Cold storage of platelets has recently regained interest because of potential advantages such as reduced bacterial growth and preserved function. However, platelets exposed to cold temperatures change uniformly from a discoid to a spherical shape, reducing the morphology score outcomes to spheroid versus discoid during cooling. We developed a simpler, unbiased screening tool to measure temperature-induced platelet shape change using imaging flow cytometry. When reduced to two dimensions, spheres appear circular, while discs are detected on a spectrum from fusiform to circular. We defined circular events as having a transverse axis of >0.8 of the longitudinal axis and fusiform events ≤0.8 of the longitudinal axis. Using this assay, mouse and human platelets show a temperature and time-dependent, two-dimensional shape change from fusiform to circular, consistent with their three-dimensional change from discs to spheres. The method we describe here is a valuable tool for detecting shape change differences in response to agonists or temperature and will help screening for therapeutic measures to mitigate the cold-induced storage lesion.
ISSN:0953-7104
1369-1635
1369-1635
DOI:10.1080/09537104.2022.2136646