Multi-omics analysis and the remedial effects of Swertiamarin on hepatic injuries caused by CCl4

Hepatic diseases pose a significant threat to community health, impacting the quality of life and longevity of millions worldwide. Despite revolutionary advancements in treatment, liver diseases remain a pressing issue, necessitating the development of more effective therapeutic approaches. Here, we...

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Published in:Ecotoxicology and environmental safety 2024-09, Vol.282, p.116734, Article 116734
Main Authors: Li, Linzhen, Xiao, Shengjia, Dai, Xiangjie, Tang, Zhiyi, Wang, Yutong, Ali, Munwar, Ataya, Farid Shokry, Sahar, Irna, Iqbal, Mujahid, Wu, Yi, Li, Kun
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CCl4
Liver damage
Metabonomics
Mice
Microbiome
Swertimarin
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creator Li, Linzhen
Xiao, Shengjia
Dai, Xiangjie
Tang, Zhiyi
Wang, Yutong
Ali, Munwar
Ataya, Farid Shokry
Sahar, Irna
Iqbal, Mujahid
Wu, Yi
Li, Kun
description Hepatic diseases pose a significant threat to community health, impacting the quality of life and longevity of millions worldwide. Despite revolutionary advancements in treatment, liver diseases remain a pressing issue, necessitating the development of more effective therapeutic approaches. Here, we conducted a comprehensive multi-omics analysis to investigate the underlying mechanism of Swertiamarin in alleviating hepatic injuries induced by CCl4 in mice. We divided 100 Kunming mice into five groups: RC (control), RM (CCl4), RD (15 mg/Kg Swertiamarin), RZ (30 mg/Kg Swertiamarin), and RG (60 mg/Kg Swertiamarin). Animals in groups RD, RZ, and RG received daily Swertiamarin via gavage, while those in groups RM, RD, RZ, and RG were treated with CCl4 solution intraperitoneally every four days, nine times in total. Our findings revealed that mice in the RM group exhibited slightly lower average weights compared to other groups, along with significantly higher liver weight (p
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Despite revolutionary advancements in treatment, liver diseases remain a pressing issue, necessitating the development of more effective therapeutic approaches. Here, we conducted a comprehensive multi-omics analysis to investigate the underlying mechanism of Swertiamarin in alleviating hepatic injuries induced by CCl4 in mice. We divided 100 Kunming mice into five groups: RC (control), RM (CCl4), RD (15 mg/Kg Swertiamarin), RZ (30 mg/Kg Swertiamarin), and RG (60 mg/Kg Swertiamarin). Animals in groups RD, RZ, and RG received daily Swertiamarin via gavage, while those in groups RM, RD, RZ, and RG were treated with CCl4 solution intraperitoneally every four days, nine times in total. Our findings revealed that mice in the RM group exhibited slightly lower average weights compared to other groups, along with significantly higher liver weight (p<0.0001) and liver index (p<0.0001). Pathological analysis indicated liver damage characterized by cell degeneration, inflammatory cell infiltration, and hepatic fibrosis in the CCl4-induced group. In contrast, Swertiamarin supplementation mitigated these effects, reducing denatured cells, inflammatory cells, and collagenous fibers in the liver. Serum analysis showed elevated levels of TNF-α (p<0.001), IL-6 (p<0.05), ALT (p<0.001), AST (p<0.0001), MDA (p<0.001), and Hyp (p<0.001) in CCl4-induced animals, along with lower levels of T-AOC (p<0.001), GSH-px (p<0.0001), SOD (p<0.001), and CAT (p<0.01). Microbiome analysis revealed significant differences among groups, with pathogenic taxa such as Arthrinium and Aureobasidium, and probiotic Saccharomyces showing notable variations. Metabolomics analysis identified numerous differentially abundant metabolites, with Swertiamarin-treated animals exhibiting distinct profiles. Our findings highlight the potential of Swertiamarin ameliorating CCl4-induced liver toxicity through modulation of antioxidant capacity, inflammatory response, gut microbiota, and metabolites. These insights may inform the development of novel therapies for liver injury. •Hepatic diseases threaten community health, affecting millions' quality of life and longevity worldwide.•Swertiamarin reduced denatured cells, inflammatory cells, and collagenous fiber in the liver.•Microbiome analysis revealed significant changed taxa like Arthrinium, Aureobasidium and Saccharomyces in mice.•Metabolomics identified differentially abundant metabolites, with Swertiamarin-treated animals exhibiting distinct profiles.]]></description><identifier>ISSN: 0147-6513</identifier><identifier>ISSN: 1090-2414</identifier><identifier>EISSN: 1090-2414</identifier><identifier>DOI: 10.1016/j.ecoenv.2024.116734</identifier><language>eng</language><publisher>Elsevier Inc</publisher><subject>CCl4 ; Liver damage ; Metabonomics ; Mice ; Microbiome ; Swertimarin</subject><ispartof>Ecotoxicology and environmental safety, 2024-09, Vol.282, p.116734, Article 116734</ispartof><rights>2024 The Authors</rights><rights>Copyright © 2024 The Authors. 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Pathological analysis indicated liver damage characterized by cell degeneration, inflammatory cell infiltration, and hepatic fibrosis in the CCl4-induced group. In contrast, Swertiamarin supplementation mitigated these effects, reducing denatured cells, inflammatory cells, and collagenous fibers in the liver. Serum analysis showed elevated levels of TNF-α (p<0.001), IL-6 (p<0.05), ALT (p<0.001), AST (p<0.0001), MDA (p<0.001), and Hyp (p<0.001) in CCl4-induced animals, along with lower levels of T-AOC (p<0.001), GSH-px (p<0.0001), SOD (p<0.001), and CAT (p<0.01). Microbiome analysis revealed significant differences among groups, with pathogenic taxa such as Arthrinium and Aureobasidium, and probiotic Saccharomyces showing notable variations. Metabolomics analysis identified numerous differentially abundant metabolites, with Swertiamarin-treated animals exhibiting distinct profiles. 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Despite revolutionary advancements in treatment, liver diseases remain a pressing issue, necessitating the development of more effective therapeutic approaches. Here, we conducted a comprehensive multi-omics analysis to investigate the underlying mechanism of Swertiamarin in alleviating hepatic injuries induced by CCl4 in mice. We divided 100 Kunming mice into five groups: RC (control), RM (CCl4), RD (15 mg/Kg Swertiamarin), RZ (30 mg/Kg Swertiamarin), and RG (60 mg/Kg Swertiamarin). Animals in groups RD, RZ, and RG received daily Swertiamarin via gavage, while those in groups RM, RD, RZ, and RG were treated with CCl4 solution intraperitoneally every four days, nine times in total. Our findings revealed that mice in the RM group exhibited slightly lower average weights compared to other groups, along with significantly higher liver weight (p<0.0001) and liver index (p<0.0001). Pathological analysis indicated liver damage characterized by cell degeneration, inflammatory cell infiltration, and hepatic fibrosis in the CCl4-induced group. In contrast, Swertiamarin supplementation mitigated these effects, reducing denatured cells, inflammatory cells, and collagenous fibers in the liver. Serum analysis showed elevated levels of TNF-α (p<0.001), IL-6 (p<0.05), ALT (p<0.001), AST (p<0.0001), MDA (p<0.001), and Hyp (p<0.001) in CCl4-induced animals, along with lower levels of T-AOC (p<0.001), GSH-px (p<0.0001), SOD (p<0.001), and CAT (p<0.01). Microbiome analysis revealed significant differences among groups, with pathogenic taxa such as Arthrinium and Aureobasidium, and probiotic Saccharomyces showing notable variations. Metabolomics analysis identified numerous differentially abundant metabolites, with Swertiamarin-treated animals exhibiting distinct profiles. Our findings highlight the potential of Swertiamarin ameliorating CCl4-induced liver toxicity through modulation of antioxidant capacity, inflammatory response, gut microbiota, and metabolites. These insights may inform the development of novel therapies for liver injury. •Hepatic diseases threaten community health, affecting millions' quality of life and longevity worldwide.•Swertiamarin reduced denatured cells, inflammatory cells, and collagenous fiber in the liver.•Microbiome analysis revealed significant changed taxa like Arthrinium, Aureobasidium and Saccharomyces in mice.•Metabolomics identified differentially abundant metabolites, with Swertiamarin-treated animals exhibiting distinct profiles.]]></abstract><pub>Elsevier Inc</pub><doi>10.1016/j.ecoenv.2024.116734</doi><oa>free_for_read</oa></addata></record>
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source ScienceDirect Freedom Collection 2022-2024; Elsevier ScienceDirect Journals
subjects CCl4
Liver damage
Metabonomics
Mice
Microbiome
Swertimarin
title Multi-omics analysis and the remedial effects of Swertiamarin on hepatic injuries caused by CCl4
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