Study on the gut symbiotic microbiota in long- and short-winged brown planthopper, Nilaparvata lugens (Stål)

• Published in: Scientific reports 2024-05, Vol.14 (1), p.11306-11306, Article 11306
• Main Authors: Zhao, Jingjing, Guan, Guangxiang, Li, Danting, Yu, Xiaoping, Shentu, Xuping
• Format: Article
• Language: English
• Subjects: 631/326; 631/337; 631/45; Adaptability; Animals; Bacteria - classification; Bacteria - genetics; Community structure; Developmental stages; Dimorphism; Female; Flora; Gastrointestinal Microbiome; Gut symbiotic microbiota; Hemiptera - microbiology; Hemiptera - physiology; High throughput sequencing; Humanities and Social Sciences; Metabolic pathways; Metagenomics; Microbiota; multidisciplinary; Muscles; Next-generation sequencing; Nilaparvata lugens; Pest control; Pests; RNA, Ribosomal, 16S - genetics; rRNA 16S; Science; Science (multidisciplinary); Symbiosis; Symbiotic bacteria diversity; Wing dimorphism; Wings; Wings, Animal - microbiology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-62350-2

The brown planthopper (BPH), Nilaparvata lugens (Stål), is one of the most important rice pests in Asia rice regions. BPH has monophagy, migration, rapid reproduction and strong environmental adaptability, and its control is a major problem in pest management. Adult BPH exhibit wing dimorphism, and the symbiotic microbiota enriched in the gut can provide energy for wing flight muscles as a source of nutrition. In order to study the diversity of symbiotic microbiota in different winged BPHs, this paper takes female BPH as the research object. It was found that the number of symbiotic microbiota of different winged BPHs would change at different development stages. Then, based on the 16S rRNA and ITS sequences, a metagenomic library was constructed, combined with fluorescent quantitative PCR and high-throughput sequencing, the dominant symbiotic microbiota flora in the gut of different winged BPHs was found, and the community structure and composition of symbiotic microbiota in different winged BPHs were further determined. Together, our results preliminarily revealed that symbiotic microbiota in the gut of BPHs have certain effects on wing morphology, and understanding the mechanisms underlying wing morph differentiation will clarify how nutritional factors or environmental cues alter or regulate physiological and metabolic pathways. These findings also establish a theoretical basis for subsequent explorations into BPH-symbiont interplay.