Intraoperative PRO Score Assessment of Actinic Keratosis with FCF Fast Green-Enhanced Ex Vivo Confocal Microscopy

Actinic keratoses (AKs) represent a common skin cancer in situ associated with chronic sun exposure. Early diagnosis and management of AKs are crucial to prevent their progression to invasive squamous cell carcinoma. Therefore, we investigated AK PRO score assessment using ex vivo confocal laser mic...

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Bibliographic Details
Published in:Applied sciences 2024-01, Vol.14 (3), p.1150
Main Authors: Hartmann, Daniela, Buttgereit, Lisa, Stärr, Lara, Sattler, Elke Christina, French, Lars Einar, Deußing, Maximilian
Format: Article
Language:English
Subjects:
actinic keratoses
bedside histology
Cancer
Care and treatment
Collagen
dermatology
Development and progression
Diagnosis
Histology
Keratosis
Lasers
Microscope and microscopy
Microscopy
Microsurgery
non-invasive imaging
reflectance confocal microscopy
Skin
skin cancer
Squamous cell carcinoma
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Summary:Actinic keratoses (AKs) represent a common skin cancer in situ associated with chronic sun exposure. Early diagnosis and management of AKs are crucial to prevent their progression to invasive squamous cell carcinoma. Therefore, we investigated AK PRO score assessment using ex vivo confocal laser microscopy (EVCM) coupled with a novel fluorescent dye, FCF Fast Green, to explore its potential for the precise imaging and discrimination of collagen fibers. AK PRO assessment using EVCM demonstrated excellent conformity (95.8%) with histopathologic examination. The additional utilization of FCF Fast Green dye had no impact on AK visualization but showed a high affinity for collagen fibers enabling clear differentiation of collagen alterations between healthy and sun-damaged skin. The enhanced visualization of collagen fiber changes may aid clinicians in accurately identifying AKs and differentiating them from benign skin lesions.
ISSN:2076-3417
2076-3417
DOI:10.3390/app14031150