Identification, Quantification, and Characterization of the Phenolic Fraction of Brunfelsia grandiflora: In Vitro Antioxidant Capacity

Brunfelsia grandiflora is an ancient plant widely used for its promising medicinal properties, although little explored scientifically. Despite being a rich source of phenolic compounds responsible in part for the proven anti-inflammatory activity, its characterization has not been carried out to da...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2022-10, Vol.27 (19), p.6510
Main Authors: Mateos, Raquel, Ramos-Cevallos, Norma, Castro-Luna, Americo, Ramos-Gonzalez, Mariella, Clavo, Zoyla-Mirella, Quispe-Solano, Miguel, Goya, Luis, Rodríguez, José-Luis
Format: Article
Language:English
Subjects:
Acids
Anti-inflammatory agents
antioxidant activity
Antioxidants
Arthritis
Bark
Brunfelsia grandiflora
Dry matter
Flavanols
Flavonols
Flowers & plants
Gallates
Herbal medicine
Hydroxycinnamic acid
hydroxycoumarins
Inflammation
LC-MS-QToF
Lignans
medicinal plant
Medicinal plants
Medicine
Narcotics
Organic solvents
Pain
Phenolic compounds
Phenols
Phytochemicals
Polyphenols
Rheumatism
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Summary:Brunfelsia grandiflora is an ancient plant widely used for its promising medicinal properties, although little explored scientifically. Despite being a rich source of phenolic compounds responsible in part for the proven anti-inflammatory activity, its characterization has not been carried out to date. The present work deals with the exhaustive identification and quantification of its phenolic fraction, along with its antioxidant activity. Decoction resulting from the bark as fine powder was filtered and lyophilized, and polyphenols were extracted from the resulting product by aqueous-organic solvents. Seventy-nine polyphenols were identified using LC-MSn. Hydroxycinnamates was the most abundant group of compounds (up to 66.8%), followed by hydroxycoumarins (15.5%), lignans (6.1%), flavonols (5.7%), phenolic simples (3.1), gallates (2.3%), flavanols (0.3%), and flavanones (0.2%). About 64% of the characterized phenols were in their glycosylated forms. The quantification of these phytochemicals by LC-QToF showed that this medicinal plant contained 2014.71 mg of phenolic compounds in 100 g dry matter, which evidences a great antioxidant potency determined by ABTS and DPPH assays. Therefore, Brunfelsia grandiflora represents an important source of polyphenols which supports its therapeutic properties scientifically proven.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules27196510