Establishment of a reverse transcription recombinase-aided amplification detection method for porcine group a rotavirus

Porcine rotavirus type A (PoRVA) is the main cause of dehydration and diarrhea in piglets, which has a great impact on the development of the pig industry worldwide. A rapid, accurate and sensitive detection method is conducive to the monitoring, control, and removal of PoRVA. In this study, a PoRVA...

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Bibliographic Details
Published in:Frontiers in veterinary science 2022-09, Vol.9, p.954657-954657
Main Authors: Wang, Yushun, Nie, Mincai, Deng, Huidan, Lai, Siyuan, Zhou, Yuancheng, Sun, Xiangan, Zhu, Ling, Xu, Zhiwen
Format: Article
Language:English
Subjects:
detection assay
porcine rotavirus
RT-qPCR methods
RT-RAA
Veterinary Science
VP6 gene
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Summary:Porcine rotavirus type A (PoRVA) is the main cause of dehydration and diarrhea in piglets, which has a great impact on the development of the pig industry worldwide. A rapid, accurate and sensitive detection method is conducive to the monitoring, control, and removal of PoRVA. In this study, a PoRVA real-time fluorescent reverse transcription recombinase-aided amplification (RT-RAA) assay was developed. Based on the PoRVA VP6 gene, specific primers and probes were designed and synthesized. The sensitivity of RT-RAA and TaqMan probe-based RT-qPCR was 7 copies per reaction and 5 copies per reaction, respectively. The sensitivity of the RT-RAA method was close to TaqMan probe-based RT-qPCR. The detection results of RT-RAA and TaqMan probe-based quantitative real-time RT-PCR methods were completely consistent in 241 clinical samples. Therefore, we successfully established a rapid and specific RT-RAA diagnostic method for PoRVA.
ISSN:2297-1769
2297-1769
DOI:10.3389/fvets.2022.954657