Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants

RNAs play important roles in regulating biological growth and development. Advancements in RNA-imaging techniques are expanding our understanding of their function. Several common RNA-labeling methods in plants have pros and cons. Simultaneously, plants' spontaneously fluorescent substances int...

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Bibliographic Details
Published in:Plants (Basel) 2024-03, Vol.13 (5), p.743
Main Authors: Yang, Zheng-Chao, Zhao, Li-Xiang, Sang, Yu-Qi, Huang, Xin, Lin, Xuan-Chen, Yu, Zhi-Ming
Format: Article
Language:English
Subjects:
aggregation-induced emission (AIE)
Amino acids
Analysis
Aqueous solutions
Artificial intelligence
Chemical reactions
Chemical synthesis
click chemistry
CRISPR
Developmental stages
Emission
Emissions
Enzymes
Fluorescence
Gene expression
Genetic engineering
Hydrocarbons
Imaging techniques
Innovations
Labeling
Liquid phases
Localization
Luminescence
Medical imaging
MicroRNAs
New technology
Optical properties
Phase separation
Plant cells
Proteins
Review
RNA
RNA aptamer
RNA fluorescence labeling
RNA modification
Substrates
Toxicity
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Summary:RNAs play important roles in regulating biological growth and development. Advancements in RNA-imaging techniques are expanding our understanding of their function. Several common RNA-labeling methods in plants have pros and cons. Simultaneously, plants' spontaneously fluorescent substances interfere with the effectiveness of RNA bioimaging. New technologies need to be introduced into plant RNA luminescence. Aggregation-induced emission luminogens (AIEgens), due to their luminescent properties, tunable molecular size, high fluorescence intensity, good photostability, and low cell toxicity, have been widely applied in the animal and medical fields. The application of this technology in plants is still at an early stage. The development of AIEgens provides more options for RNA labeling. Click chemistry provides ideas for modifying AIEgens into RNA molecules. The CRISPR/Cas13a-mediated targeting system provides a guarantee of precise RNA modification. The liquid-liquid phase separation in plant cells creates conditions for the enrichment and luminescence of AIEgens. The only thing that needs to be looked for is a specific enzyme that uses AIEgens as a substrate and modifies AIEgens onto target RNA via a click chemical reaction. With the development and progress of artificial intelligence and synthetic biology, it may soon be possible to artificially synthesize or discover such an enzyme.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants13050743