Alcohol induced behavioral and immune perturbations are attenuated by activation of CB2 cannabinoid receptors

The endocannabinoidome (eCBome) is the expanded endocannabinoid system (ECS) and studies show that there is a link between this system and how it modulates alcohol induced neuroinflammation. Using conditional knockout (cKO) mice with selective deletion of cannabinoid type 2 receptors (CB2Rs) in dopa...

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Bibliographic Details
Published in:Advances in Drug and Alcohol Research 2023-12, Vol.3, p.11602-11602
Main Authors: Roberts, Aaliyah, Christian, Mahli, Dilone, Lizbeth Nivar, Nelson, Natania, Endrino, Mark Joseph, Kneebone, Adam, Embaby, Shymaa, Fernandez, Justin, Liu, Qing-Rong, Onaivi, Emmanuel S, Kibret, Berhanu Geresu
Format: Article
Language:English
Subjects:
alcohol
CB2Rs
eCBome
ECS
Health Archive
inflammation
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Summary:The endocannabinoidome (eCBome) is the expanded endocannabinoid system (ECS) and studies show that there is a link between this system and how it modulates alcohol induced neuroinflammation. Using conditional knockout (cKO) mice with selective deletion of cannabinoid type 2 receptors (CB2Rs) in dopamine neurons (DAT- ) and in microglia (Cx3Cr1- ), we investigated how CB2Rs modulate behavioral and neuroinflammation induced by alcohol. Behavioral tests including locomotor and wheel running activity, rotarod performance test, and alcohol preference tests were used to evaluate behavioral changes induced by alcohol. Using ELISA assay, we investigated the level of pro-inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1α (IL-1α), and interleukin-1β (IL-1β) in the hippocampus of mice. The findings demonstrated that locomotor activity, wheel running, and rotarod performance activities were significantly affected by cell-type specific deletion of CB2Rs in dopamine neurons and microglia. The non-selective CB2R agonist, WIN 55,212-2, reduced alcohol preference in the wild type and cell-type specific CB2R cKO mice. In addition, the result showed that cell-type specific deletion of CB2Rs and administration of alcohol to CB2R cKO mice increased the expression of proinflammatory cytokines in the hippocampus. These findings suggest the involvement of CB2Rs in modulating behavioral and immune alterations induced by alcohol.
ISSN:2674-0001
2674-0001
DOI:10.3389/adar.2023.11602