Migration Groups: A Poorly Explored Point of View for Genetic Damage Assessment Using Comet Assay in Human Lymphocytes

A new point of view for genetic damage assessment using the comet assay is proposed based on the number of migration groups, the number of comets in each group, and the groups with the highest number of comets. Human lymphocytes were exposed to different concentrations of Methyl Methane Sulfonate (M...

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Bibliographic Details
Published in:Applied sciences 2021-05, Vol.11 (9), p.4094
Main Authors: Reynoso-Silva, Mónica, Álvarez-Moya, Carlos, Ramírez-Velasco, Rafael, Sámano-León, Alexis Gerardo, Arvizu-Hernández, Erandi, Castañeda-Vásquez, Hugo, Ruíz-Lopez, Mario Alberto
Format: Article
Language:English
Subjects:
2,4-D
bioassays
Chromosome aberrations
Comet assay
Damage assessment
Damage detection
Deoxyribonucleic acid
DNA
DNA damage
Genotoxic chemicals
Genotoxicity
human lymphocytes
Lymphocytes
Maleic hydrazide
Software
Statistical analysis
tail length groups
Toxicity
Variance analysis
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Summary:A new point of view for genetic damage assessment using the comet assay is proposed based on the number of migration groups, the number of comets in each group, and the groups with the highest number of comets. Human lymphocytes were exposed to different concentrations of Methyl Methane Sulfonate (MMS), Maleic Hydrazide (MH), 2,4-Dichlorophenoxyacetic (2,4-D), and N-nitroso diethylamine (NDEA). Using comet assay, the migration means of the comets were determined and later grouped arbitrarily in migration groups with no higher differences than 1 µc. The number of migration groups, the number of comets in each group, and the groups with the highest number of comets (modes) were determined. All four of the genotoxic agents studied showed a significant increase (p < 0.05) in the tail length and the number of migration groups compared to the negative control. The number of migration groups did not show a significant variation between the four-genotoxic agents nor within their different concentrations. However, the comparison of the modes did show differences between the genotoxic agents, but not within the concentrations of a same genotoxic agent, which indicated a determined chemical interaction on the DNA. These parameters can improve the detection of genetic damage associated with certain genotoxic agents.
ISSN:2076-3417
2076-3417
DOI:10.3390/app11094094