DnaK response to expression of protein mutants is dependent on translation rate and stability

Chaperones play a central part in the quality control system in cells by clearing misfolded and aggregated proteins. The chaperone DnaK acts as a sensor for molecular stress by recognising short hydrophobic stretches of misfolded proteins. As the level of unfolded protein is a function of protein st...

Full description

Saved in:
Bibliographic Details
Published in:Communications biology 2022-06, Vol.5 (1), p.597-597, Article 597
Main Authors: Christensen, Signe, Rämisch, Sebastian, André, Ingemar
Format: Article
Language:English
Subjects:
13/31
631/337/470/1981
631/45/470/1981
82/80
96/35
Biochemistry and Molecular Biology
Biokemi och molekylärbiologi
Biologi
Biological Sciences
Biology
Biomedical and Life Sciences
DnaK protein
Homeostasis
Hydrophobicity
Life Sciences
Mutants
Natural Sciences
Naturvetenskap
Protein biosynthesis
Protein folding
Proteins
Quality control
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Chaperones play a central part in the quality control system in cells by clearing misfolded and aggregated proteins. The chaperone DnaK acts as a sensor for molecular stress by recognising short hydrophobic stretches of misfolded proteins. As the level of unfolded protein is a function of protein stability, we hypothesised that the level of DnaK response upon overexpression of recombinant proteins would be correlated to stability. Using a set of mutants of the λ-repressor with varying thermal stabilities and a fluorescent reporter system, the effect of stability on DnaK response and protein abundance was investigated. Our results demonstrate that the initial DnaK response is largely dependent on protein synthesis rate but as the recombinantly expressed protein accumulates and homeostasis is approached the response correlates strongly with stability. Furthermore, we observe a large degree of cell-cell variation in protein abundance and DnaK response in more stable proteins. A survey of λ-repressor mutants using a fluorescent reporter system reveals the chaperone DnaK response for protein quality control.
ISSN:2399-3642
2399-3642
DOI:10.1038/s42003-022-03542-2