Nuclear Magnetic Resonance Treatment Induces ßNGF Release from Schwann Cells and Enhances the Neurite Growth of Dorsal Root Ganglion Neurons In Vitro

• Published in: Cells (Basel, Switzerland) Switzerland), 2024-09, Vol.13 (18), p.1544
• Main Authors: Rad, Anda, Weigl, Lukas, Steinecker-Frohnwieser, Bibiane, Stadlmayr, Sarah, Millesi, Flavia, Haertinger, Maximilian, Borger, Anton, Supper, Paul, Semmler, Lorenz, Wolf, Sonja, Naghilou, Aida, Weiss, Tamara, Kress, Hans G, Radtke, Christine
• Format: Article
• Language: English
• Subjects: Adenosine triphosphate; Animals; Antibodies; Axonogenesis; Blocking antibodies; Calcium (intracellular); Calcium channels; Capsaicin; Capsaicin receptors; Cell culture; Cells, Cultured; Culture Media, Conditioned - pharmacology; Cytokines; Dorsal root ganglia; DRG neuron; Enzyme-linked immunosorbent assay; Fibroblasts; Ganglia, Spinal - drug effects; Ganglia, Spinal - metabolism; Ganglion; Glial Cell Line-Derived Neurotrophic Factor - metabolism; Growth; Growth factors; Intracellular signalling; low nuclear magnetic resonance therapy; Muscle proteins; Nerve growth factor; neurite outgrowth; Neurites - drug effects; Neurites - metabolism; Neurons; Neurons - drug effects; Neurons - metabolism; NMR; Nuclear magnetic resonance; Pain perception; Penicillin; peripheral nerve regeneration; Peripheral nerves; Potassium channels; Rats; Rats, Wistar; Regeneration; Schwann cell; Schwann cells; Schwann Cells - drug effects; Schwann Cells - metabolism; Transient receptor potential proteins; TRPV Cation Channels - metabolism; Viral antibodies
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells13181544

Peripheral nerve regeneration depends on close interaction between neurons and Schwann cells (SCs). After nerve injury, SCs produce growth factors and cytokines that are crucial for axon re-growth. Previous studies revealed the supernatant of SCs exposed to nuclear magnetic resonance therapy (NMRT) treatment to increase survival and neurite formation of rat dorsal root ganglion (DRG) neurons in vitro. The aim of this study was to identify factors involved in transferring the observed NMRT-induced effects to SCs and consequently to DRG neurons. Conditioned media of NMRT-treated (CM NMRT) and untreated SCs (CM CTRL) were tested by beta-nerve growth factor (ßNGF) ELISA and multiplex cytokine panels to profile secreted factors. The expression of nociceptive transient receptor potential vanilloid 1 (TRPV1) channels was assessed and the intracellular calcium response in DRG neurons to high-potassium solution, capsaicin or adenosine triphosphate was measured mimicking noxious stimuli. NMRT induced the secretion of ßNGF and pro-regenerative-signaling factors. Blocking antibody experiments confirmed ßNGF as the main factor responsible for neurotrophic/neuritogenic effects of CM NMRT. The TRPV1 expression or sensitivity to specific stimuli was not altered, whereas the viability of cultured DRG neurons was increased. Positive effects of CM NMRT supernatant on DRG neurons are primarily mediated by increased ßNGF levels.