Water Jacket Systems for Temperature Control of Petri Dish Cell Culture Chambers

Water jacket systems are routinely used to control the temperature of Petri dish cell culture chambers. Despite their widespread use, the thermal characteristics of such systems have not been fully investigated. In this study, we conducted a comprehensive set of theoretical, numerical and experiment...

Full description

Saved in:
Bibliographic Details
Published in:Applied sciences 2019-02, Vol.9 (4), p.621
Main Authors: Uharek, Samira, Baratchi, Sara, Zhu, Jiu, Alshehri, Majed, Mitchell, Arnan, Rebner, Karsten, Karnutsch, Christian, Khoshmanesh, Khashayar
Format: Article
Language:English
Subjects:
Aluminum
Cell culture
Cell differentiation
Cell proliferation
Chambers
Control systems
Convection
convective heaters
Cooling
Differentiation (biology)
Environmental monitoring
Enzymatic activity
Free convection
Gene expression
Heat conductivity
Heat transfer
Infrared cameras
Monitoring
Petri dish
Polystyrene resins
Process parameters
Protein transport
Reservoirs
Rubber
Temperature control
Thermocouples
water jacket
Water jackets
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Water jacket systems are routinely used to control the temperature of Petri dish cell culture chambers. Despite their widespread use, the thermal characteristics of such systems have not been fully investigated. In this study, we conducted a comprehensive set of theoretical, numerical and experimental analyses to investigate the thermal characteristics of Petri dish chambers under stable and transient conditions. In particular, we investigated the temperature gradient along the radial axis of the Petri dish under stable conditions, and the transition period under transient conditions. Our studies indicate a radial temperature gradient of 3.3 °C along with a transition period of 27.5 min when increasing the sample temperature from 37 to 45 °C for a standard 35 mm diameter Petri dish. We characterized the temperature gradient and transition period under various operational, geometric, and environmental conditions. Under stable conditions, reducing the diameter of the Petri dish and incorporating a heater underneath the Petri dish can effectively reduce the temperature gradient across the sample. In comparison, under transient conditions, reducing the diameter of the Petri dish, reducing sample volume, and using glass Petri dish chambers can reduce the transition period.
ISSN:2076-3417
2076-3417
DOI:10.3390/app9040621