Leaf Mutant 7 Encoding Heat Shock Protein OsHSP40 Regulates Leaf Size in Rice

Leaf size is an important agronomic trait directly affecting yield in rice, and thus understanding the genes determining leaf size is important in breeding. In this study, one ( ) with small leaf size was isolated using ethyl methane sulphonate (EMS) mutagenesis from the Zhenggeng 1925. MutMap by wh...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-04, Vol.23 (8), p.4446
Main Authors: Wang, Fuhua, Tang, Zhengbin, Wang, Ya, Fu, Jing, Yang, Wenbo, Wang, Shengxuan, Wang, Yuetao, Bai, Tao, Huang, Zhibo, Yin, Haiqing, Wang, Zhoufei
Format: Article
Language:English
Subjects:
Abiotic stress
Cell division
Cell number
Cell size
Chromosome 7
Cloning
Ethyl methanesulfonate
Genes
Genomes
Genotype & phenotype
Heat
heat shock protein
Heat shock proteins
Heat-Shock Proteins - genetics
Heat-Shock Proteins - metabolism
leaf size
Leaves
Mutagenesis
Mutants
natural variation
Nucleotides
Oryza - genetics
Oryza - metabolism
Phenotype
Plant Breeding
Plant growth
Plant Leaves - genetics
Plant Leaves - metabolism
Polymorphism
Polymorphism, Single Nucleotide
Population
Rice
Single-nucleotide polymorphism
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Summary:Leaf size is an important agronomic trait directly affecting yield in rice, and thus understanding the genes determining leaf size is important in breeding. In this study, one ( ) with small leaf size was isolated using ethyl methane sulphonate (EMS) mutagenesis from the Zhenggeng 1925. MutMap by whole genome resequencing of phenotypic bulks revealed that is likely located in the 133 kb region on chromosome 7 using F population from a cross between and wild-type (WT) Zhenggeng 1925. The candidate gene encoding heat shock protein for was functionally validated. Disruption of this gene in mutants significantly reduced the leaf size compared with that of WT in rice. Microscopic examination showed that modulated leaf size via regulating the veins formation and cell size/cell number. Nucleotide diversity analysis indicated that a single nucleotide polymorphism (SNP) variation of C to T in the coding region of may cause small leaves among rice accessions. Therefore, the natural variation of contributing to leaf size might be useful for rice breeding.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23084446