Tandem Mass Tag-Based Serum Proteome Profiling for Biomarker Discovery in Young Duchenne Muscular Dystrophy Boys

Blood-accessible molecular biomarkers are becoming highly attractive tools to assess disease progression and response to therapies in Duchenne muscular dystrophy (DMD) especially in very young patients for whom other outcome measures remain subjective and challenging. In this study, we have standard...

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Bibliographic Details
Published in:ACS omega 2020-10, Vol.5 (41), p.26504-26517
Main Authors: Alayi, Tchilabalo D., Tawalbeh, Shefa M., Ogundele, Michael, Smith, Holly R., Samsel, Alison M., Barbieri, Marissa L., Hathout, Yetrib
Format: Article
Language:English
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Summary:Blood-accessible molecular biomarkers are becoming highly attractive tools to assess disease progression and response to therapies in Duchenne muscular dystrophy (DMD) especially in very young patients for whom other outcome measures remain subjective and challenging. In this study, we have standardized a highly specific and reproducible multiplexing mass spectrometry method using the tandem mass tag (TMT) strategy in combination with depletion of abundant proteins from serum and high-pH reversed-phase peptide fractionation. Differential proteome profiling of 4 year-old DMD boys ( n = 9) and age-matched healthy controls ( n = 9) identified 38 elevated and 50 decreased serum proteins (adjusted P < 0.05, FDR
ISSN:2470-1343
2470-1343
DOI:10.1021/acsomega.0c03206