Immunomodulatory effects of mesenchymal stem cell-conditioned media on lipopolysaccharide of Vibrio cholerae as a vaccine candidate

Vibrio cholerae is the causative agent of cholera, which is commonly associated with high morbidity and mortality, and presents a major challenge to healthcare systems throughout the world. Lipopolysaccharide (LPS) is required for full protection against V. cholerae but can induce inflammation and s...

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Bibliographic Details
Published in:Stem cell research & therapy 2021-11, Vol.12 (1), p.564-564, Article 564
Main Authors: Bahroudi, Mahboube, Bakhshi, Bita, Soudi, Sara, Najar-Peerayeh, Shahin
Format: Article
Language:English
Subjects:
Analysis
Animal models
Animals
Antibodies
Antibodies, Bacterial
Antibody
Antigens
Antimicrobial agents
BLyS protein
Cholera
Cholera toxin
Culture Media, Conditioned - pharmacology
Cytokine
Cytokines
Cytotoxicity
Drug dosages
Drug resistance
Flow cytometry
Humans
Immune response
Immunity
Immunization
Immunoglobulin A
Immunoglobulin G
Immunomodulation
Inflammation
Inflammatory diseases
Interleukin 10
Interleukin 4
Interleukin 5
Interleukin 6
Lipopolysaccharides
Lipopolysaccharides - pharmacology
LPS
Lymphocytes B
Medical research
Medicine, Experimental
Mesenchymal Stem Cells
Mice
Mitogens
Morbidity
Saliva
Septic shock
Stem cell transplantation
Stem cells
Transforming growth factors
Tumor necrosis factor-α
Vaccines
Vibrio cholerae
Vibrio cholerae O1
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Summary:Vibrio cholerae is the causative agent of cholera, which is commonly associated with high morbidity and mortality, and presents a major challenge to healthcare systems throughout the world. Lipopolysaccharide (LPS) is required for full protection against V. cholerae but can induce inflammation and septic shock. Mesenchymal stem cells (MSCs) are currently used to treat infectious and inflammatory diseases. Therefore, this study aimed to evaluate the immune-modulating effects of the LPS-MSC-conditioned medium (CM) on V. cholerae LPS immunization in a murine model. After preconditioning MSCs with LPS, mice were immunized intraperitoneally on days 0 and 14 with the following combinations: LPS + LPS-MSC-CM; detoxified LPS (DLPS) + MSC-CM; LPS + MSC sup; LPS; LPS-MSC-CM; MSC supernatant (MSC sup); and PBS. The mouse serum and saliva samples were collected to evaluate antibody (serum IgG and saliva IgA) and cytokine responses (TNF-α, IL-10, IL-6, TGF-β, IL-4, IL-5, and B-cell activating factor (BAFF)). The LPS + LPS-MSC-CM significantly increased total IgG and IgA compared to other combinations (P 
ISSN:1757-6512
1757-6512
DOI:10.1186/s13287-021-02622-0