A Novel Strategy for Unveiling Spatial Distribution Pattern of Gallotannins in Paeonia rockii and Paeonia ostii Based on LC-QTRAP-MS

Gallotannins (GTs) are a series of hydrolyzable tannins with multiple health-promoting effects. In this study, an integrated liquid chromatography tandem mass spectrometry (LC-MS/MS) strategy was developed for unveiling the spatial distribution pattern of GTs in the emerging oilseed crops and . Acco...

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Bibliographic Details
Published in:Metabolites 2022-04, Vol.12 (4), p.326
Main Authors: Bai, Zhangzhen, Yu, Rui, Zheng, Tiantian, Sun, Daoyang, Zhou, Yang, Tang, Junman, Zhu, Huili, Li, Guangning, Niu, Lixin, Cui, Lu, Du, Rui, Zhang, Jing, Zhang, Yanlong
Format: Article
Language:English
Subjects:
Chromatography
Efficiency
Flowers & plants
gallotannins
Glucose
Identification
Ions
Libraries
Liquid chromatography
liquid chromatography-quadrupole ion trap-mass spectrometry (LC–QTRAP–MS)
Mass spectroscopy
Metabolites
multiple reaction monitoring
neutral loss
NMR
Nuclear magnetic resonance
Oilseeds
Paeonia rockii
Protocol
Seeds
Software
Spatial distribution
spatial distribution pattern
tree peony
Trees
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Summary:Gallotannins (GTs) are a series of hydrolyzable tannins with multiple health-promoting effects. In this study, an integrated liquid chromatography tandem mass spectrometry (LC-MS/MS) strategy was developed for unveiling the spatial distribution pattern of GTs in the emerging oilseed crops and . According to the fragmentation behavior of the representative GT (1,2,3,4,6-penta- -galloyl-β- -glucose, PGG), the diagnostic neutral loss (NL) of 170 Da was chosen for the non-targeted screening of GT precursors. Simultaneously, the tandem mass spectrometry spectrum (MS/MS) information was acquired through an enhanced product ion (EPI) scan. Nine major GTs were identified in tree peony. To quantify the targeted GTs in different tissues of tree peony, we established a multiple reaction monitoring (MRM)-enhanced product ion (EPI)-based pseudo-targeted approach under the information-dependent acquisition (IDA) mode. The quantitative results show that the GT compounds were ubiquitous in tree peony plants with diverse structures. The typical GT PGG was mainly distributed in roots, leaves, and petals. This strategy can also be utilized for metabolite characterization and quantification in other substrates.
ISSN:2218-1989
2218-1989
DOI:10.3390/metabo12040326