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Exploratory insights from the immuno-oncology hollow fiber assay: A pilot approach bridging In Vitro and In Vivo models
•An in vivo Hollow Fiber Assay for immuno-oncology drug testing.•Time and cost-efficient in vivo evaluation on cancer-and immune cells of human origin.•Co-cultures grown in semipermeable hollow fibers implanted in mice.•Monitoring of immune-cell mediated cancer cell killing in multiple fibers per mo...
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Published in: | SLAS technology 2024-12, Vol.29 (6), p.100232, Article 100232 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | •An in vivo Hollow Fiber Assay for immuno-oncology drug testing.•Time and cost-efficient in vivo evaluation on cancer-and immune cells of human origin.•Co-cultures grown in semipermeable hollow fibers implanted in mice.•Monitoring of immune-cell mediated cancer cell killing in multiple fibers per mouse.•Potential to reduce and refine animal use in immuno-oncology research.
To facilitate the translation of immunotherapies from bench to bedside, predictive preclinical models are essential. We developed the in vivo immuno-oncology Hollow Fiber Assay (HFA) to bridge the gap between simpler cell-based in vitro assays and more complex mouse models for immuno-oncology drug evaluation. The assay involves co-culturing human cancer cell lines or primary patient-derived cancer cells with human immune cells inside semipermeable hollow fibers. Implanted intraperitoneally in mice, the fibers captured treatment-induced immune cell-mediated cancer cell killing following treatments with aCD3 and/or IL-2, demonstrating the feasibility of the assay. Traditional models require lengthy observation periods to monitor tumor growth and treatment response. The immuno-oncology HFA enables a rapid initial in vivo evaluation of immunological agents on cancer and immune cells of human origin, addressing two of the 3Rs — reduction and refinement — in animal research. |
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ISSN: | 2472-6303 2472-6311 2472-6311 |
DOI: | 10.1016/j.slast.2024.100232 |