Essential factors for in vitro regeneration of rose and a protocol for plant regeneration from leaves

In vitro propagation of Rosa hybrida, L. cv. ‘Eiffel Tower’ was improved by the addition of thidiazuron (TDZ) and silver nitrate (AgNo3) to the culture medium. The combination of auxin and cytokinins was indispensable for inducing response from leaf discs. Maintaining cultures under dark was better...

Full description

Saved in:
Bibliographic Details
Published in:Horticultural science (Praha) 2018-01, Vol.45 (2), p.83-91
Main Authors: Mahmoud, Inas Mohamed Ali, Hassanein, Anber Mahmoud Ahmed
Format: Article
Language:English
Subjects:
Acetic acid
Adenine
Butyric acid
Callus
Cytokinins
direct regeneration
Elongation
embryogenesis
Embryonic growth stage
Embryos
Explants
Gibberellic acid
Indole-3-butyric acid
leaf discs
Leaves
organogenesis
Plants
Propagation
Regeneration
Rooting
rosa hybrida
Shoots
Silver
Silver nitrate
Somatic embryogenesis
Thidiazuron
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In vitro propagation of Rosa hybrida, L. cv. ‘Eiffel Tower’ was improved by the addition of thidiazuron (TDZ) and silver nitrate (AgNo3) to the culture medium. The combination of auxin and cytokinins was indispensable for inducing response from leaf discs. Maintaining cultures under dark was better than light for callus formation and quality. The source of explants was vital in the regeneration process wherein situ explants produced callus while, in vitro explants regenerated somatic embryos and shoots. Gibberellic acid (GA3) had a favorable effect where in vitro explants showed somatic embryogenesis with no shoots on media containing TDZ however, 37% of explants regenerated shoots directly on medium containing GA3. The presence of benzyl adenine (BA) was essential for shoot elongation, and indole butyric acid (IBA) was better than indole acetic acid (IAA) for rooting. The optimum conditions produced rooted plants from leaf discs within ten weeks. The reported results clarify factors controlling in vitro regeneration of R. hybrida, and provide a rapid protocol allowing further improvements of rose.
ISSN:0862-867X
1805-9333
DOI:10.17221/12/2017-HORTSCI