Identification of Phlorotannins in the Brown Algae, Saccharina latissima and Ascophyllum nodosum by Ultra-High-Performance Liquid Chromatography Coupled to High-Resolution Tandem Mass Spectrometry

Phlorotannins are bioactive polyphenols in brown macroalgae that make these algae interesting as healthy food. Specific phlorotannins are, however, seldom identified, and extracts from different species are often only analysed for total phenolic content (TPC). In this study, our focus was to identif...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2020-12, Vol.26 (1), p.43
Main Authors: Sardari, Roya R R, Prothmann, Jens, Gregersen, Olavur, Turner, Charlotta, Nordberg Karlsson, Eva
Format: Article
Language:English
Subjects:
Analytical Chemistry
Analytisk kemi
Ascophyllum - chemistry
Ascophyllum nodosum
Brown algae
Chemical Sciences
Chromatography, High Pressure Liquid - methods
Extraction
High-resolution mass spectrometry
Kemi
Natural Sciences
Naturvetenskap
Phaeophyceae - chemistry
Phlorotannins
Polyphenols
Saccharina latissima
Tandem Mass Spectrometry - methods
Tannins - analysis
Tannins - chemistry
Tannins - isolation & purification
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Summary:Phlorotannins are bioactive polyphenols in brown macroalgae that make these algae interesting as healthy food. Specific phlorotannins are, however, seldom identified, and extracts from different species are often only analysed for total phenolic content (TPC). In this study, our focus was to identify phlorotannin molecules from and (a species rich in these compounds) using ultra-high-performance liquid chromatography coupled to high-resolution tandem mass spectrometry (UHPLC-HRMS ). Water and ethanol (30 and 80% / ) were used at solid:liquid ratios, extraction times and temperatures, proposed to result in high TPC in extracts from other species. The extracts, however, did not allow phlorotannin detection by either UHPLC-UV/Vis or UHPLC-HRMS , despite a TPC response by the Folin-Ciocalteu assay, pinpointing a problem with interference by non-phenolic compounds. Purification by solid phase extraction (SPE) led to purer, more concentrated fractions and identification of four phlorotannin species in and one in by UHPLC-HRMS , using extracts in ethanol 80% / at a solid:liquid ratio of 1:10 for 20 h at 25 °C with an added 10 h at 65 °C incubation of remaining solids. The phlorotannin with the formula C H O (corresponding to bifuhalol) is the first identified in .
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26010043