Ondas ultrassônicas e pérolas de vidro: um novo método de extração de beta-galactosidase para uso em laboratório Ultrasonic waves and glass pearls: a new method of extraction of beta-galactosodade for use in laboratory

The optimization of a traditional technique of cellular disruption by abrasion was carried out and a process using ultrasonic waves associated with glass pearls to extract beta-galactosidase from Kluyveromyces marxianus proposed. In the first case, the effects of the diameter and weight of the pearl...

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Bibliographic Details
Published in:Química nova 2008-01, Vol.31 (2), p.336-339
Main Authors: Fabiana Oliveira de Medeiros, Fernanda Germano Alves, Cristiane Reinaldo Lisboa, Daniela de Souza Martins, Carlos André Veiga Burkert, Susana Juliano Kalil
Format: Article
Language:English
Subjects:
beta-galactosidase
cellular disruption
sonification
Online Access:Get full text
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Summary:The optimization of a traditional technique of cellular disruption by abrasion was carried out and a process using ultrasonic waves associated with glass pearls to extract beta-galactosidase from Kluyveromyces marxianus proposed. In the first case, the effects of the diameter and weight of the pearls in relation to the volume of cellular suspension and amount of time for cellular disruption were evaluated. The efficiency of the new process of cellular disruption was evaluated by varying the length of time of sonification and comparing with the method of abrasion under the same conditions. The proposed method can be efficiently applied to obtain beta-galactosidase at laboratory scale.
ISSN:0100-4042
1678-7064
DOI:10.1590/S0100-40422008000200028