Bromelain reduced pro-inflammatory mediators as a common pathway that mediate antinociceptive and anti-anxiety effects in sciatic nerve ligated Wistar rats

• Published in: Scientific reports 2021-01, Vol.11 (1), p.289-289, Article 289
• Main Authors: Bakare, Ahmed O., Owoyele, Bamidele V.
• Format: Article
• Language: English
• Subjects: 631/443; 631/443/376; 692/617; 692/699; Animal models; Anxiety; Cerebral cortex; Enzyme-linked immunosorbent assay; Filaments; Humanities and Social Sciences; Hyperalgesia; IL-1β; Immunohistochemistry; Inflammation; Interleukin 6; multidisciplinary; Nitric-oxide synthase; Pain perception; Peripheral neuropathy; Prostaglandin E2; Rodents; Sciatic nerve; Science; Science (multidisciplinary); Tumor necrosis factor-α
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-79421-9

The involvement of pro-inflammatory mediators complicates the complex mechanism in neuropathic pain (NP). This study investigated the roles of bromelain against pro-inflammatory mediators as a mechanism that underpins its antinociceptive and anti-anxiety effects in the peripheral model of NP. Sixty-four male Wistar rats randomly divided into eight groups, were used for the study. A chronic constriction injury model of peripheral neuropathy was used to induce NP. Tail-immersion and von Frey filaments tests were used to assess hyperalgesia while open field and elevated plus mazes were used to assess anxiety-like behaviour. NF-кB, iNOS, nitrate, and pro-inflammatory cytokines were investigated in the plasma, sciatic nerve, and brain tissues using ELISA, spectrophotometer, and immunohistochemistry techniques after twenty-one days of treatment. Bromelain significantly (p