Intestinal Dysbiosis in Autoimmune Diabetes Is Correlated With Poor Glycemic Control and Increased Interleukin-6: A Pilot Study

• Published in: Frontiers in immunology 2018-07, Vol.9, p.1689-1689
• Main Authors: Higuchi, Bruna Stevanato, Rodrigues, Nathália, Gonzaga, Marina Ignácio, Paiolo, João Carlos Cicogna, Stefanutto, Nadine, Omori, Wellington Pine, Pinheiro, Daniel Guariz, Brisotti, João Luiz, Matheucci, Jr, Euclides, Mariano, Vânia Sammartino, de Oliveira, Gislane Lelis Vilela
• Format: Article
• Language: English
• Subjects: dietary habits; glycemic control; Immunology; inflammatory cytokines; interleukin-6; intestinal dysbiosis; type 1 diabetes
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2018.01689

Intestinal dysbiosis associated with immunological deregulation, leaky gut, bacterial translocation, and systemic inflammation has been associated with autoimmune diseases, such as type 1 diabetes (T1D). The aim of this study was to investigate the intestinal dysbiosis in T1D patients and correlate these results with clinical parameters and cytokines. The present study was approved by the Barretos Cancer Hospital (Process number 903/2014), and all participants have signed the informed consent in accordance with the Declaration of Helsinki, and answered a questionnaire about dietary habits. Stool samples were used for bacterial 16S sequencing by MiSeq Illumina platform. IL-2, IL-4, IL-6, IL-10, IL-17A, TNF, and IFN-γ plasma concentrations were determined by cytometric bead arrays. The Pearson's chi-square, Mann-Whitney and Spearman correlation were used for statistical analyses. Alpha and beta diversities were conducted by using an annotated observed taxonomic units table. This study included 20 patients and 28 controls, and we found significant differences (