Osteogenic actions of the osteogenic growth peptide on bovine marrow mesenchymal stromal cells in culture

• Published in: Veterinární medicína 2008-09, Vol.53 (9), p.501-509
• Main Authors: Zhu, H.B.,Huazhong Agricultural Univ., Wuhan (China). Coll. of Veterinary Medicine, Guo, D.Z.,Huazhong Agricultural Univ., Wuhan (China). Coll. of Veterinary Medicine, Yang, S.J.,Huazhong Agricultural Univ., Wuhan (China). Coll. of Veterinary Medicine, Zhang, Y.H.,Huazhong Agricultural Univ., Wuhan (China). Coll. of Veterinary Medicine, Wang, H.,Huazhong Agricultural Univ., Wuhan (China). Coll. of Veterinary Medicine, Guo, H.T.,Huazhong Agricultural Univ., Wuhan (China). Coll. of Veterinary Medicine, Zhang, Y.,Huazhong Agricultural Univ., Wuhan (China). Coll. of Veterinary Medicine, Cheng, D.C.,Huazhong Agricultural Univ., Wuhan (China). Coll. of Veterinary Medicine
• Format: Article
• Language: English
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; ALKALINE PHOSPHATASE; ARN; Biomedical materials; BONE MARROW; bovine; CELL CULTURE; Cell differentiation; Cell lines; CELLS; CELLULE; CELULAS; CRECIMIENTO; CROISSANCE; CULTIVO DE CELULAS; CULTIVO IN VITRO; Culture; CULTURE DE CELLULE; CULTURE IN VITRO; Differentiation (biology); enos; ENZYME ACTIVITY; EXPRESION GENICA; EXPRESSION DES GENES; FOSFATASA ALCALINA; GENE; GENE EXPRESSION; GENES; GROWTH; http://www.fao.org/aos/agrovoc#c_1418; http://www.fao.org/aos/agrovoc#c_15999; http://www.fao.org/aos/agrovoc#c_24135; http://www.fao.org/aos/agrovoc#c_2604; http://www.fao.org/aos/agrovoc#c_267; http://www.fao.org/aos/agrovoc#c_27527; http://www.fao.org/aos/agrovoc#c_3214; http://www.fao.org/aos/agrovoc#c_3394; http://www.fao.org/aos/agrovoc#c_34079; http://www.fao.org/aos/agrovoc#c_36996; http://www.fao.org/aos/agrovoc#c_4320; http://www.fao.org/aos/agrovoc#c_5691; http://www.fao.org/aos/agrovoc#c_6618; http://www.fao.org/aos/agrovoc#c_7178; http://www.fao.org/aos/agrovoc#c_998; IN VITRO CULTURE; LIGASAS; LIGASE; LIGASES; marrow stromal cells; MEDULA OSEA; Mesenchyme; MINERALISATION; MINERALIZACION; MINERALIZATION; MOELLE OSSEUSE; NITRIC OXIDE; Osteoblasts; Osteocalcin; osteogenic growth peptide; OXIDO NITRICO; OXYDE NITRIQUE; PCR; PEPTIDE; PEPTIDES; PEPTIDOS; PHOSPHATASE ALCALINE; RNA; Stem cell transplantation; Stem cells; Stromal cells
• ISSN: 0375-8427; 1805-9392
• DOI: 10.17221/1981-vetmed

The results of this study aiming to evaluate the effects of the osteogenic growth peptide (OGP) on the growth and differentiation of bovine marrow mesenchymal stem cells in culture show that OGP promotes osteogenic differentiation of the bovine stem cells. OGP increased alkaline phosphatase activity and mineralized nodule formation, and stimulated osteoblast-specific mRNA expression of Osteocalcin. On the other hand, OGP dose-dependently stimulated the expression of endothelial nitric oxide synthases. These results show for the first time a direct osteogenic effect of OGP on bovine marrow stromal cells in culture.