Oral probiotic activities and biosafety of Lactobacillus gasseri HHuMIN D

Lactobacillus spp. have been researched worldwide and are used in probiotics, but due to difficulties with laboratory cultivation of and experimentation on oral microorganisms, there are few reports of Lactobacillus spp. being isolated from the oral cavity and tested against oral pathogens. This res...

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Bibliographic Details
Published in:Microbial cell factories 2021-03, Vol.20 (1), p.75-75, Article 75
Main Authors: Mann, Soyon, Park, Myeong Soo, Johnston, Tony V, Ji, Geun Eog, Hwang, Keum Taek, Ku, Seockmo
Format: Article
Language:English
Subjects:
Adhesion
Age
Anaerobic microorganisms
Antimicrobial agents
Antimicrobial Effect
Bacteria
Biosafety
Biosafety Evaluation
Care and treatment
Cell adhesion
Cell culture
Dental caries
Dental plaque
Dentifrices
Dilution
Disease prevention
Experimentation
Food
Halitosis
Hydrogen peroxide
Lactobacillus
Lactobacillus gasseri
Microbiological research
Microbiology
Microorganisms
Mouth
Mouth diseases
Off odor
Oral cavity
Oral diseases
Oral hygiene
Oral hygiene products
Oral Microorganisms
Probiotics
Quality control
Safety
Starter cultures
Sucrose
Testing
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Summary:Lactobacillus spp. have been researched worldwide and are used in probiotics, but due to difficulties with laboratory cultivation of and experimentation on oral microorganisms, there are few reports of Lactobacillus spp. being isolated from the oral cavity and tested against oral pathogens. This research sought to isolate and determine the safety and inhibitory capabilities of a Lactobacillus culture taken from the human body. One organism was isolated, named "L. gasseri HHuMIN D", and evaluated for safety. A 5% dilution of L. gasseri HHuMIN D culture supernatant exhibited 88.8% inhibition against halitosis-producing anaerobic microorganisms and the organism itself exhibited powerful inhibitory effects on the growth of 11 oral bacteria. Hydrogen peroxide production reached 802 μmol/L after 12 h and gradually diminished until 24 h, it efficiently aggregated with P. catoniae and S. sanguinis, and it completely suppressed S. mutans-manufactured artificial dental plaque. L. gasseri HHuMIN D's KB cell adhesion capacity was 4.41 cells per cell, and the cell adhesion of F. nucleatum and S. mutans diminished strongly in protection and displacement assays. These results suggest that L. gasseri HHuMIN D is a safe, bioactive, lactobacterial food ingredient, starter culture, and/or probiotic microorganism for human oral health.
ISSN:1475-2859
1475-2859
DOI:10.1186/s12934-021-01563-w