Anti-Wrinkling and Anti-Melanogenic Effect of Pradosia mutisii Methanol Extract

• Published in: International journal of molecular sciences 2019-02, Vol.20 (5), p.1043
• Main Authors: Lorz, Laura Rojas, Yoo, Byong Chul, Kim, Mi-Yeon, Cho, Jae Youl
• Format: Article
• Language: English
• Subjects: Aging; anti-melanogenic effect; anti-wrinkling effect; antioxidant activity; Bioactive compounds; Chromatography; Collagen; Curcumin; Enzymes; Epicatechin; Flowers; Free radicals; Gallic acid; Genes; Hydration; Hydrogen peroxide; Hydroxycinnamic acid; Hyperpigmentation; Kinases; Melanin; Metabolites; moisturizing; Oxidative stress; Proteins; Rosmarinic acid; Scavenging; Skin; Sulfonic acid; Tyrosinase; Tyrosol
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20051043

Ultraviolet (UV) exposure causes skin photoaging leading to skin wrinkling and sagging via production of reactive oxygen species (ROS). For this reason, protection from photoaging is an important feature in cosmeceutical and dermatological products. Natural product-derived biomaterials are highly desired as future possible ingredients, because these biomaterials are often safe and effective. In this study, we aimed to characterize the skin protective activity of , traditionally used to treat sunburn and erythema. We determined the free radical scavenging, anti-melanogenic, and moisturizing effects of a methanol extract of (Pm-ME) in keratinocytes (HaCaT cells), melanocytes (B16F10 cells), and fibroblasts (human dermal fibroblasts (HDFs)) at non-cytotoxic concentrations. methanol extract contains coumaric acid as a major component, and the extract exhibited protective activity against UVB- and H₂O₂-induced cytotoxicity. This extract also suppressed the expression of ( s) and in HaCaT cells. A reduction of expression under UVB- and H₂O₂-treated conditions was recovered in HaCaT cells by Pm-ME. This extract displayed significant free radical scavenging activity according to the 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) assay. The Pm-ME also upregulated the expression levels of ( ) and ( ) in HaCaT cells, indicating a putative moisturizing activity. Interestingly, the expression of collagen type 1 ( ) gene and its promoter activity, as assessed by a reporter gene assay, were found to be increased in HDF and HEK293 cells. Similarly, Pm-ME helped recover collagen levels after UVB and H₂O₂ treatment in HDFs as well as decreased the synthesis and secretion of melanin from B16F10 melanoma cells, which may indicate a beneficial whitening cosmetic value. The p38 inhibitor SB203580 and the JNK inhibitor SP600125 suppressed and expression in H₂O₂-treated HaCaT cells. Similarly, the ERK inhibitor U0126 inhibited in Pm-ME/H₂O₂-treated HaCaT cells. These findings suggested that inhibition of JNK and p38 and activation of ERK could be targeted by Pm-ME. Therefore, Pm-ME may exert anti-photoaging and anti-melanogenic properties via the regulation of mitogen-activated protein kinase, which could be beneficial in the cosmeceutical industry.