Differentiation of Human Adipose-Derived Stem Cells into Endometrial Epithelial Cells

• Published in: Reproductive and developmental medicine 2020-07, Vol.4 (3), p.137-145
• Main Authors: Yang, Fang, Zhang, Wan-Lin, Chen, Shu-Qiang, Sun, Hui-Jun, Lu, Jie, Xiao, Xi-Feng, Wang, Xiao-Hong
• Format: Article
• Language: English
• Subjects: adipose-derived stem cell; endometrial epithelial cell; estrogen receptor α; estrogen receptor β; h19; β-catenin
• ISSN: 2589-8728; 2096-2924; 2589-8728
• DOI: 10.4103/2096-2924.296547

Objective: This study aimed to investigate the differentiation of human adipose-derived stem cells (hASCs) into endometrial epithelial cells (EECs) under certain induction conditions and to a further step provide a promising approach for ASCs in clinical practice to the treatment of severe intrauterine adhesion. Methods: Four groups of hASCs were separately cultured as follows: in Group 1, hASCs were cultured in a control medium (5% fetal bovine serum [FBS] + α-minimum Eagle's medium [α-MEM]); in Group 2, hASCs were cultured in an induction medium (5% FBS + α-MEM + [1 × 10−7 mol/L 17β-estradiol] + 10 ng/mL transforming growth factor β1 [TGF-β1] + 10 ng/mL epidermal growth factor [EGF] + 10 ng/mL platelet-derived growth factor BB [PDGF-BB]); in Group 3, hASCs and human endometrium cells (hEMCs) were cocultured in the control medium; and in Group 4, hASCs and hEMCs were cocultured in the induction medium. Results: When cocultured with hEMCs, the morphology of hASCs became similar with EECs, and the addition of factors such as EGF, TGFβ, PDGF-BB, and 17β-estradiol promoted differentiation. This study, for the first time, demonstrated estrogen receptor (ER)α and ERβ expression in hASCs and preliminarily explored changes in ERα, ERβ, β-catenin, and H19 mRNA expression during hASC differentiation. Furthermore, we concluded that H19 mRNA expression was negatively correlated with differentiation, which is seemingly related to the estrogen signaling pathway. Conclusions: hASCs revealed the potential for differentiating to EECs when cocultured with hEMCs.