Effect of Sucrose Concentration on Rhaponticum carthamoides (Willd.) Iljin Transformed Root Biomass, Caffeoylquinic Acid Derivative, and Flavonoid Production

• Published in: International journal of molecular sciences 2022-11, Vol.23 (22), p.13848
• Main Authors: Skała, Ewa, Olszewska, Monika Anna, Makowczyńska, Joanna, Kicel, Agnieszka
• Format: Article
• Language: English
• Subjects: 1,4,5-O-tricaffeoylquinic acid; 3,5-di-O-caffeoylquinic acid; 4,5-di-O-caffeoylquinic acid; Accumulation; Acids; Biomass; Biosynthesis; Caffeoylquinic acid; Carbohydrates; Chlorogenic acid; Dry weight; Enzymes; Flavonoids; Flowers & plants; Genetic engineering; growth cycle; Herbal medicine; Leuzea; Metabolic pathways; Metabolism; Metabolites; Osmotic stress; Phenolic acids; Phenols; Raw materials; Roots; Sucrose
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms232213848

(Willd.) Iljin is a rare, pharmacopoeial, and medicinal plant, endemic to Siberia and endangered due to the massive collection of raw material from the natural habitat. The aim of the current study was to estimate the effect of sucrose concentration (0-7%) on transformed root growth and on caffeoylquinic acid derivative (CQA) and flavonoid production. Sucrose in higher concentrations may induce osmotic stress and thus may affect secondary metabolism in plants. It was revealed that sucrose concentration influenced transformed root biomass and modified the phenolic compound metabolic pathway. However, the dynamics of both processes varied significantly. The optimal sucrose level was different for biomass accumulation and the biosynthesis of specialized metabolite. The highest dry weight of roots was achieved for 7% sucrose (31.17 g L of dry weight), while 1% sucrose was found to be optimal for phenolic acid and flavonoid production. Considering the dry weight increase and metabolite accumulation, 3% sucrose was revealed to give optimal yields of CQAs (511.1 mg L ) and flavonoids (38.9 mg L ). Chlorogenic acid, 3,5-, 4,5-di- -caffeoylquinic acids, 1,4,5- -tricaffeoylquinic acid, and a tentatively-identified tricaffeoylquinic acid derivative 1 were found to be the most abundant specialized metabolites among the identified CQAs. Our findings indicate that transformed roots may be an efficient source of CQA derivatives, with valuable health-promoting activities.