Pseudovirus Nanoparticles Displaying Plasmodium Circumsporozoite Proteins Elicited High Titers of Sporozoite-Binding Antibody

Background: malaria caused by Plasmodium parasites remains a public health threat. The circumsporozoite proteins (CSPs) of Plasmodium sporozoite play a key role in Plasmodium infection, serving as an excellent vaccine target. Methods: using a self-assembled S60 nanoparticle platform, we generated ps...

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Bibliographic Details
Published in:Vaccines (Basel) 2023-11, Vol.11 (11), p.1650
Main Authors: Xia, Ming, Huang, Pengwei, Vago, Frank, Jiang, Wen, Tan, Ming
Format: Article
Language:English
Subjects:
Antibodies
Antibody response
Antigens
Antiparasitic agents
Binding
Chromatography
Circumsporozoite protein
Drug resistance
Glycan
Health risks
Heparan sulfate
Immunization
Immunogenicity
Infections
Insecticides
Liver
Malaria
malaria vaccine
Mosquitoes
Nanoparticles
Parasites
Plasmodium
Proteins
Public health
Receptors
S60 nanoparticle
Self-assembly
Sporozoites
Three dimensional models
Vaccines
Vector-borne diseases
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Summary:Background: malaria caused by Plasmodium parasites remains a public health threat. The circumsporozoite proteins (CSPs) of Plasmodium sporozoite play a key role in Plasmodium infection, serving as an excellent vaccine target. Methods: using a self-assembled S60 nanoparticle platform, we generated pseudovirus nanoparticles (PVNPs) displaying CSPs, named S-CSPs, for enhanced immunogenicity. Results: purified Hisx6-tagged or tag-free S-CSPs self-assembled into PVNPs that consist of a norovirus S60 inner shell and multiple surface-displayed CSPs. The majority of the PVNPs measured ~27 nm with some size variations, and their three-dimensional structure was modeled. The PVNP-displayed CSPs retained their glycan receptor-binding function. A mouse immunization study showed that PVNPs induced a high antibody response against CSP antigens and the PVNP-immunized mouse sera stained the CSPs of Plasmodium sporozoites at high titer. Conclusions and discussion: the PVNP-displayed CSPs retain their authentic antigenic feature and receptor-binding function. The CSP-specific antibody elicited by the S-CSP PVNPs binds original CSPs and potentially inhibits the attachment of Plasmodium sporozoites to their host cells, a key step for liver invasion by the sporozoites. Thus, S-CSP PVNPs may be an excellent vaccine candidate against malaria caused by Plasmodium parasites.
ISSN:2076-393X
2076-393X
DOI:10.3390/vaccines11111650