Purification and Characterization of Antibacterial Activity against Phytopathogenic Bacteria in Culture Fluids from Ganoderma lucidum

Previous studies of have focused on its medicinal applications. Limited information is available about its antibacterial activity against plant pathogens. Thus, the goal of this study was to purify and characterize the antibacterial activity against plant pathogenic bacteria from culture fluids of T...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2021-09, Vol.26 (18), p.5553
Main Authors: Robles-Hernández, Loreto, Salas-Salazar, Nora A, Gonzalez-Franco, Ana C
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - isolation & purification
Anti-Bacterial Agents - pharmacology
Antibacterial activity
Antibacterial materials
antibacterial polysaccharides
Antimicrobial agents
Bacteria
Bacteria - drug effects
basidiomycetes
Bioactive compounds
Biological activity
Chromatography
Chromatography, Gel
Chromatography, High Pressure Liquid
Dialysis
Ganoderma lucidum
Gas Chromatography-Mass Spectrometry
GC-MS
Glucose
Gram-negative bacteria
Gram-positive bacteria
Heat
Hemodialysis
High performance liquid chromatography
HPLC-APCI-MS
Lipids
Liquid chromatography
Microbial Sensitivity Tests
Molecular Weight
Mushrooms
Organic solvents
Pathogens
Polysaccharides
Polysaccharides - chemistry
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
Proteinase
Reishi - chemistry
Saccharides
Sterols
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Summary:Previous studies of have focused on its medicinal applications. Limited information is available about its antibacterial activity against plant pathogens. Thus, the goal of this study was to purify and characterize the antibacterial activity against plant pathogenic bacteria from culture fluids of The nature of the bioactive components was determined using heat boiling, organic solvents, dialysis tubing, gel exclusion chromatography (GEC), proteinase sensitivity, HPLC, HPLC-APCI-MS, and GC-MS. The bioactive compounds were neither lipid, based on their solubility, nor proteic in nature, based on proteinase digestion and heat stability. The putative-bioactive polysaccharides have molecular weights that range from 3500 to 4500 Daltons as determined by dialysis tubing, GEC and APCI-MS analysis. The composition of the antibacterial compounds was determined by GC-MS. This is the first report of small polysaccharides produced by with activity against bacterial plant pathogens.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26185553