Direct intra-tumoral injection of zinc-acetate halts tumor growth in a xenograft model of prostate cancer

Intracellular levels of zinc have shown a strong inverse correlation to growth and malignancy of prostate cancer. To date, studies of zinc supplementation in prostate cancer have been equivocal and have not accounted for bioavailability of zinc. Therefore, we hypothesized that direct intra-tumoral i...

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Published in:Journal of experimental & clinical cancer research 2009-06, Vol.28 (1), p.84-84, Article 84
Main Authors: Shah, Maulik R, Kriedt, Christopher L, Lents, Nathan H, Hoyer, Mary K, Jamaluddin, Nimah, Klein, Claudette, Baldassare, Joseph
Format: Article
Language:English
Subjects:
Animal models in research
Animals
Care and treatment
Cell Proliferation
Diagnosis
Health aspects
Humans
Injections, Intralesional
Male
Mice
Mice, Inbred NOD
Mice, SCID
Neoplasms, Hormone-Dependent - drug therapy
Neoplasms, Hormone-Dependent - pathology
Prostate cancer
Prostatic Neoplasms - drug therapy
Prostatic Neoplasms - pathology
Tumor Cells, Cultured
Xenograft Model Antitumor Assays
Zinc Acetate - administration & dosage
Zinc compounds
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Summary:Intracellular levels of zinc have shown a strong inverse correlation to growth and malignancy of prostate cancer. To date, studies of zinc supplementation in prostate cancer have been equivocal and have not accounted for bioavailability of zinc. Therefore, we hypothesized that direct intra-tumoral injection of zinc could impact prostate cancer growth. In this study, we evaluated the cytotoxic properties of the pH neutral salt zinc acetate on the prostate cancer cell lines PC3, DU145 and LNCaP. Zinc acetate killed prostate cancer cell lines in vitro, independent of androgen sensitivity, in a dose-dependent manner in a range between 200 and 600 microM. Cell death occurred rapidly with 50% cell death by six hours and maximal cell death by 18 hours. We next established a xenograft model of prostate cancer and tested an experimental treatment protocol of direct intra-tumoral injection of zinc acetate. We found that zinc treatments halted the growth of the prostate cancer tumors and substantially extended the survival of the animals, whilst causing no detectable cytoxicity to other tissues. Thus, our studies form a solid proof-of-concept that direct intra-tumoral injection of zinc acetate could be a safe and effective treatment strategy for prostate cancer.
ISSN:1756-9966
0392-9078
1756-9966
DOI:10.1186/1756-9966-28-84