Induced neural stem/progenitor cell‐derived extracellular vesicles promote recovery post‐stroke

• Published in: Clinical and translational medicine 2022-06, Vol.12 (6), p.e936-n/a
• Main Authors: Gao, Ge, Li, Congcong, Zhu, Jie, Sheng, Shiyang, Liang, Zhanping, Fu, Shengyang, Li, Xiangyu, Sun, Yiyan, Wang, Yi, Feng, Xuanran, Xia, Xiaohuan, Zheng, Jialin C.
• Format: Article
• Language: English
• Subjects: Cognitive ability; Growth factors; Kinases; Letter to Editor; Neurogenesis; Phosphorylation; Stem cells; Traumatic brain injury
• ISSN: 2001-1326; 2001-1326
• DOI: 10.1002/ctm2.936

The significantly higher proportions of NeuN+ cells might also indicate the neuroprotection effects of EVs in the initial phase of post-stroke. [...]both EVs enhance neurogenesis post MCAO, and iNPC-EVs may be with higher efficacy in promoting NPC proliferation (Figure 2B) and neuroregeneration in the MCAO brain (Figure 2D–F). [...]both behavioral test and histological/molecular studies suggested outstanding therapeutic potential of both NPC- and iNPC-EVs in treating IS without significant adverse effect. Since promising results have been reported for the clinical safety of clinical-grade EVs,8 the production and clinical safety of clinical-grade iNPC-EVs are required to be investigated in our future study. SEE PDF] We then carried out preliminary studies to identify the potential mechanisms underlying the iNPC-EV-mediated recovery post MCAO. Since our proteomic analysis has implied enrichment of growth factor domains in iNPC-EVs,6 we examined the protein levels of multiple growth factors that are commonly expressed in the brain cells in EVs. Both EVs activated MEK-ERK pathway, but not PI3K-AKT pathway, in both hippocampal and cortical tissues, ascertained by the up-regulation of MEK and ERK phosphorylation (Figure S7). [...]iNPC-EVs are with stronger promoting effects on the activities of MEK-ERK pathway than NPC-EVs (Figure S7).