Characterization of TSET, an ancient and widespread membrane trafficking complex

The heterotetrameric AP and F-COPI complexes help to define the cellular map of modern eukaryotes. To search for related machinery, we developed a structure-based bioinformatics tool, and identified the core subunits of TSET, a 'missing link' between the APs and COPI. Studies in Dictyostel...

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Published in:eLife 2014-05, Vol.3, p.e02866
Main Authors: Hirst, Jennifer, Schlacht, Alexander, Norcott, John P, Traynor, David, Bloomfield, Gareth, Antrobus, Robin, Kay, Robert R, Dacks, Joel B, Robinson, Margaret S
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Bioinformatics
Biology
Cell Biology
Cell Membrane - metabolism
clathrin
Computational Biology
Databases, Genetic
Datasets
Dictyostelium - genetics
Dictyostelium - metabolism
Eukaryotes
Evolution, Molecular
FCHo
Gene Expression Regulation
Genomes
Genomics
Genomics and Evolutionary Biology
membrane traffic
Membrane trafficking
Membrane turnover
muniscin
Naegleria - genetics
Organisms
Phylogenetics
Phylogeny
Protein Conformation
Protein Transport
Proteins
Proteomics
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Sequence Analysis, DNA
TPC
TPLATE
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Summary:The heterotetrameric AP and F-COPI complexes help to define the cellular map of modern eukaryotes. To search for related machinery, we developed a structure-based bioinformatics tool, and identified the core subunits of TSET, a 'missing link' between the APs and COPI. Studies in Dictyostelium indicate that TSET is a heterohexamer, with two associated scaffolding proteins. TSET is non-essential in Dictyostelium, but may act in plasma membrane turnover, and is essentially identical to the recently described TPLATE complex, TPC. However, whereas TPC was reported to be plant-specific, we can identify a full or partial complex in every eukaryotic supergroup. An evolutionary path can be deduced from the earliest origins of the heterotetramer/scaffold coat to its multiple manifestations in modern organisms, including the mammalian muniscins, descendants of the TSET medium subunits. Thus, we have uncovered the machinery for an ancient and widespread pathway, which provides new insights into early eukaryotic evolution.DOI: http://dx.doi.org/10.7554/eLife.02866.001.
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.02866