Recombinant protein expression in Escherichia coli: advances and challenges

Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of...

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Bibliographic Details
Published in:Frontiers in microbiology 2014-04, Vol.5, p.172-172
Main Authors: Rosano, Germán L, Ceccarelli, Eduardo A
Format: Article
Language:English
Subjects:
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E. coli expression strains
Escherichia coli
expression plasmid
Inclusion Bodies
Microbiology
recombinant protein production
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Summary:Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies. We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2014.00172