Cyclic increase in the ADAMTS1-L1CAM-EGFR axis promotes the EMT and cervical lymph node metastasis of oral squamous cell carcinoma

• Published in: Cell death & disease 2024-01, Vol.15 (1), p.82-16, Article 82
• Main Authors: Chien, Ming-Hsien, Yang, Yi-Chieh, Ho, Kuo-Hao, Ding, Yi-Fang, Chen, Li-Hsin, Chiu, Wen-Kuan, Chen, Ji-Qing, Tung, Min-Che, Hsiao, Michael, Lee, Wei-Jiunn
• Format: Article
• Language: English
• Subjects: 13/109; 13/44; 13/51; 13/89; 45/41; 631/67/1536/1665; 631/80/84/2176; 631/80/84/750; 64/60; 82/29; 82/80; 96/95; ADAMTS-1 protein; ADAMTS1 Protein; Animals; Antibodies; Apigenin; Biochemistry; Biomedical and Life Sciences; Carcinoma, Squamous Cell; Cell adhesion molecules; Cell Biology; Cell Culture; Down-regulation; Epidermal growth factor receptors; Epithelial-Mesenchymal Transition; ErbB Receptors; Flavonoids; Genomes; Head and neck carcinoma; Head and Neck Neoplasms; Humans; Immunology; Invasiveness; Life Sciences; Lymph nodes; Lymphatic Metastasis; Lymphatic system; Matrix metalloproteinase; Metastases; Metastasis; Mice; Mouth Neoplasms; Neural Cell Adhesion Molecule L1; Oral cancer; Oral carcinoma; Oral squamous cell carcinoma; Patients; Plants; Prognosis; Signal transduction; Squamous cell carcinoma; Squamous Cell Carcinoma of Head and Neck; Thrombospondin; Tumors; Xenografts
• ISSN: 2041-4889; 2041-4889
• DOI: 10.1038/s41419-024-06452-9

The matrix metalloprotease A disintegrin and metalloprotease with thrombospondin motifs 1 (ADAMTS1) was reported to be involved in tumor progression in several cancer types, but its contributions appear discrepant. At present, the role of ADAMTS1 in oral squamous cell carcinoma (SCC; OSCC) remains unclear. Herein, The Cancer Genome Atlas (TCGA) database showed that ADAMTS1 transcripts were downregulated in head and neck SCC (HNSCC) tissues compared to normal tissues, but ADAMTS1 levels were correlated with poorer prognoses of HNSCC patients. In vitro, we observed that ADAMTS1 expression levels were correlated with the invasive abilities of four OSCC cell lines, HSC-3, SCC9, HSC-3M, and SAS. Knockdown of ADAMTS1 in OSCC cells led to a decrease and its overexpression led to an increase in cell-invasive abilities in vitro as well as tumor growth and lymph node (LN) metastasis in OSCC xenografts. Mechanistic investigations showed that the cyclic increase in ADAMTS1-L1 cell adhesion molecule (L1CAM) axis-mediated epidermal growth factor receptor (EGFR) activation led to exacerbation of the invasive abilities of OSCC cells via inducing epithelial-mesenchymal transition (EMT) progression. Clinical analyses revealed that ADAMTS1, L1CAM, and EGFR levels were all correlated with worse prognoses of HNSCC patients, and patients with ADAMTS1 high /L1CAM high or EGFR high tumors had the shortest overall and disease-specific survival times. As to therapeutic aspects, we discovered that an edible plant-derived flavonoid, apigenin (API), drastically inhibited expression of the ADAMTS1-L1CAM-EGFR axis and reduced the ADAMTS1-triggered invasion and LN metastasis of OSCC cells in vitro and in vivo. Most importantly, API treatment significantly prolonged survival rates of xenograft mice with OSCC. In summary, ADAMTS1 may be a useful biomarker for predicting OSCC progression, and API potentially retarded OSCC progression by targeting the ADAMTS1-L1CAM-EGFR signaling pathway.