Proteomic Methods of Detection and Quantification of Protein Toxins

Biological toxins are a heterogeneous group of compounds that share commonalities with biological and chemical agents. Among them, protein toxins represent a considerable, diverse set. They cover a broad range of molecular weights from less than 1000 Da to more than 150 kDa. This review aims to comp...

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Published in:Toxins 2018-02, Vol.10 (3), p.99
Main Authors: Duracova, Miloslava, Klimentova, Jana, Fucikova, Alena, Dresler, Jiri
Format: Article
Language:English
Subjects:
analytical methods
Animals
Bacteria
Bacterial Proteins - analysis
Bacterial Proteins - toxicity
bio-terrorism
Bioassays
Biological & chemical terrorism
Biomonitoring
Chemical agents
Disease control
Humans
Identification methods
Immunoassays
In vitro methods and tests
Mass spectrometry
Mass spectroscopy
Microorganisms
Plant Proteins - analysis
Plant Proteins - toxicity
protein toxins
Proteins
proteomic
Proteomics - methods
Review
Ricin
Toxins
Toxins, Biological - analysis
Toxins, Biological - toxicity
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container_start_page 99
container_title Toxins
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creator Duracova, Miloslava
Klimentova, Jana
Fucikova, Alena
Dresler, Jiri
description Biological toxins are a heterogeneous group of compounds that share commonalities with biological and chemical agents. Among them, protein toxins represent a considerable, diverse set. They cover a broad range of molecular weights from less than 1000 Da to more than 150 kDa. This review aims to compare conventional detection methods of protein toxins such as in vitro bioassays with proteomic methods, including immunoassays and mass spectrometry-based techniques and their combination. Special emphasis is given to toxins falling into a group of selected agents, according to the Centers for Disease Control and Prevention, such as , toxins, toxins, epsilon toxin, ricin from , Abrin from or control of trade in dual-use items in the European Union, including lesser known protein toxins such as Viscumin from . The analysis of protein toxins and monitoring for biological threats, i.e., the deliberate spread of infectious microorganisms or toxins through water, food, or the air, requires rapid and reliable methods for the early identification of these agents.
doi_str_mv 10.3390/toxins10030099
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source Publicly Available Content (ProQuest); IngentaConnect Journals; PubMed Central
subjects analytical methods
Animals
Bacteria
Bacterial Proteins - analysis
Bacterial Proteins - toxicity
bio-terrorism
Bioassays
Biological & chemical terrorism
Biomonitoring
Chemical agents
Disease control
Humans
Identification methods
Immunoassays
In vitro methods and tests
Mass spectrometry
Mass spectroscopy
Microorganisms
Plant Proteins - analysis
Plant Proteins - toxicity
protein toxins
Proteins
proteomic
Proteomics - methods
Review
Ricin
Toxins
Toxins, Biological - analysis
Toxins, Biological - toxicity
title Proteomic Methods of Detection and Quantification of Protein Toxins
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