Proteomic profiling of cerebrospinal fluid reveals TKT as a potential biomarker for medulloblastoma

Cerebrospinal fluid (CSF) plays an important role in brain tumors, including medulloblastoma (MBL). Recent advancements in mass spectrometry systems and ‘Omics’ data analysis methods enable unbiased, high proteome depth research. We conducted proteomic profiling of the total CSF in MBL patients with...

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Bibliographic Details
Published in:Scientific reports 2024-09, Vol.14 (1), p.21053-14, Article 21053
Main Authors: Kim, Joo Whan, Choi, Seung Ah, Dan, Kisoon, Koh, Eun Jung, Ha, Saehim, Phi, Ji Hoon, Kim, Kyung Hyun, Han, Dohyun, Kim, Seung-Ki
Format: Article
Language:English
Subjects:
631/45/475
631/67/1857
631/67/1922
631/67/2332
Adolescent
Bioinformatics
Biomarkers
Biomarkers, Tumor - cerebrospinal fluid
Brain cancer
Brain research
Brain tumors
Cellular biology
Cerebellar Neoplasms - cerebrospinal fluid
Cerebrospinal fluid
Child
Child, Preschool
Data analysis
Extracellular vesicles
Extracellular Vesicles - metabolism
Female
Hospitals
Humanities and Social Sciences
Humans
Male
Mass spectrometry
Mass spectroscopy
Medulloblastoma
Medulloblastoma - cerebrospinal fluid
Meninges
multidisciplinary
Neurosurgery
Patients
Pediatrics
Protein seeding
Proteins
Proteome
Proteomes
Proteomics
Proteomics - methods
Science
Science (multidisciplinary)
Scientific imaging
Tumor Protein, Translationally-Controlled 1
Tumors
Citations: Items that this one cites
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Description
Summary:Cerebrospinal fluid (CSF) plays an important role in brain tumors, including medulloblastoma (MBL). Recent advancements in mass spectrometry systems and ‘Omics’ data analysis methods enable unbiased, high proteome depth research. We conducted proteomic profiling of the total CSF in MBL patients with the purpose of finding a potential diagnostic biomarker for MBL. We quantified 1112 proteins per CSF sample. Feature selection identified four elevated soluble proteins (SPTBN1, HSP90AA1, TKT, and NME1-NME2) in MBL CSF. Validation with ELISA confirmed that TKT was significantly elevated in MBL. Additionally, TKT-positive extracellular vesicles were significantly enriched in MBL CSF and correlated with the burden of leptomeningeal seeding. Our results provide insights into the proteomics data of the total CSF of MBL patients. Furthermore, we identified the significance of TKT within the total CSF and its presence within circulating EVs in the CSF. We suggest that TKT may serve as a biomarker for MBL.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-71738-z