De novo production of the flavonoid naringenin in engineered Saccharomyces cerevisiae

• Published in: Microbial cell factories 2012-12, Vol.11 (1), p.155-155, Article 155
• Main Authors: Koopman, Frank, Beekwilder, Jules, Crimi, Barbara, van Houwelingen, Adele, Hall, Robert D, Bosch, Dirk, van Maris, Antonius J A, Pronk, Jack T, Daran, Jean-Marc
• Format: Article
• Language: English
• Subjects: 3-Deoxy-7-Phosphoheptulonate Synthase - antagonists & inhibitors; 3-Deoxy-7-Phosphoheptulonate Synthase - metabolism; Acids; Acyltransferases - genetics; Acyltransferases - metabolism; Amino acids; Ammonia; Ammonia-Lyases - genetics; Ammonia-Lyases - metabolism; Antioxidants; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis thaliana; Aromatics; Biology; Bioreactors; Carbon; Carboxy-Lyases - antagonists & inhibitors; Carboxy-Lyases - metabolism; Cell culture; Comparative analysis; copy number; de novo; Dextrose; E coli; Enzymes; Feedback inhibition; Flavanones - biosynthesis; Flavonoids; Flavonoids - biosynthesis; Genetically engineered microorganisms; Genetics; Glucose; Isoflavones; Metabolic Engineering; Metabolites; Microbiology; Naringenin; Naringenin-chalcone synthase; pH effects; Phenylpyruvate decarboxylase; Phosphates; Physiological aspects; Plant Proteins - genetics; Plant Proteins - metabolism; Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - antagonists & inhibitors; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Tyrosine; Yeast
• ISSN: 1475-2859; 1475-2859
• DOI: 10.1186/1475-2859-11-155

Flavonoids comprise a large family of secondary plant metabolic intermediates that exhibit a wide variety of antioxidant and human health-related properties. Plant production of flavonoids is limited by the low productivity and the complexity of the recovered flavonoids. Thus to overcome these limitations, metabolic engineering of specific pathway in microbial systems have been envisaged to produce high quantity of a single molecules. Saccharomyces cerevisiae was engineered to produce the key intermediate flavonoid, naringenin, solely from glucose. For this, specific naringenin biosynthesis genes from Arabidopsis thaliana were selected by comparative expression profiling and introduced in S. cerevisiae. The sole expression of these A. thaliana genes yielded low extracellular naringenin concentrations (