Selection against BALB/c strain cells in mouse chimaeras

It has been shown previously that BALB/c strain embryos tend to contribute poorly to mouse aggregation chimaeras. In the present study we showed that BALB/c cells were not preferentially allocated to any extraembryonic lineages of mouse aggregation chimaeras, but their contribution decreased during...

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Bibliographic Details
Published in:Biology open 2018-01, Vol.7 (1), p.bio030189-bio030189
Main Authors: Tang, Pin-Chi, MacKay, Gillian E, Flockhart, Jean H, Keighren, Margaret A, Kopakaki, Anna, West, John D
Format: Article
Language:English
Subjects:
Agglomeration
Balb/c cells
BALB/c mouse strain
Cell selection
Chimaera
Chimera
Chimeras
Developmental stages
Embryos
Experiments
Hypotheses
Medical research
Mouse embryo
Placenta
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Summary:It has been shown previously that BALB/c strain embryos tend to contribute poorly to mouse aggregation chimaeras. In the present study we showed that BALB/c cells were not preferentially allocated to any extraembryonic lineages of mouse aggregation chimaeras, but their contribution decreased during the early postimplantation period and they were significantly depleted by E8.5. The development of BALB/c strain preimplantation embryos lagged behind embryos from some other strains and the contribution that BALB/c and other embryos made to chimaeras correlated with their developmental stage at E2.5. This relationship suggests that the poor contribution of BALB/c embryos to aggregation chimaeras is at least partly a consequence of generalised selection related to slow or delayed preimplantation development. The suitability of BALB/c embryos for maximising the ES cell contribution to mouse ES cell chimaeras is also discussed.
ISSN:2046-6390
2046-6390
DOI:10.1242/bio.030189