Determination of Kanamycin by High Performance Liquid Chromatography

Kanamycin is an aminoglycoside antibiotic widely used in treating animal diseases caused by Gram-negative and Gram-positive infections. Kanamycin has a relatively narrow therapeutic index, and can accumulate in the human body through the food chain. The abuse of kanamycin can have serious side-effec...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2019-05, Vol.24 (10), p.1902
Main Authors: Zhang, Xingping, Wang, Jiujun, Wu, Qinghua, Li, Li, Wang, Yun, Yang, Hualin
Format: Article
Language:English
Subjects:
Abuse
Aminoglycoside antibiotics
Aminoglycosides
Analytical methods
Animal diseases
Animal health
Antibiotics
Bioaccumulation
Chromatography
different detectors
Electrochemical analysis
Electrochemistry
Fluorescence
Food
Food chains
food contamination
High performance liquid chromatography
HPLC
Kanamycin
Light scattering
Liquid chromatography
Liquid-liquid extraction
Mass spectrometry
Mass spectroscopy
Proteins
Public health
Reproducibility
Retention
Review
sample pre-treatment
Solid phases
Solids
Solvents
Sorbents
Ultraviolet radiation
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Summary:Kanamycin is an aminoglycoside antibiotic widely used in treating animal diseases caused by Gram-negative and Gram-positive infections. Kanamycin has a relatively narrow therapeutic index, and can accumulate in the human body through the food chain. The abuse of kanamycin can have serious side-effects. Therefore, it was necessary to develop a sensitive and selective analysis method to detect kanamycin residue in food to ensure public health. There are many analytical methods to determine kanamycin concentration, among which high performance liquid chromatography (HPLC) is a common and practical tool. This paper presents a review of the application of HPLC analysis of kanamycin in different sample matrices. The different detectors coupled with HPLC, including Ultraviolet (UV)/Fluorescence, Evaporative Light Scattering Detector (ELSD)/Pulsed Electrochemical Detection (PED), and Mass Spectrometry, are discussed. Meanwhile, the strengths and weaknesses of each method are compared. The pre-treatment methods of food samples, including protein precipitation, liquid-liquid extraction (LLE), and solid-phase extraction (SPE) are also summarized in this paper.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24101902